抗体

β半乳糖苷酶抗体

200-4136-0100

100

https://www.rockland.com/search/?searchString=200-4136-0100

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化

抗乙GALACTOSIDASE(大肠杆菌)(兔)抗体-200-4136

β半乳糖苷酶抗体

Anti-Beta-Gal Antibody has been tested by ELISA and western blot and is suitable for dot blot, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis.   A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ.  In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 µm) to produce free-floating sections.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

多克隆

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100

微克

1:10,000

1:1,500

User Optimized

1:5, 000 - 1:10, 000

Expiration date is one (1) year from date of receipt.

IgG

lacZ

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Full length native Beta Galactosidase isolated from E.coli

Ambient

冻干

0.01%(w/v)叠氮化钠

Beta-Galactosidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.coli]. Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.

Restore with deionized water (or equivalent)

100微升

b-GAL

酶联免疫吸附测定;免疫印迹

IF; IHC; Multiplex

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

rabbit anti-Beta Galactosidase Antibody, rabbit anti-beta gal antibody, ß-Gal, Anti-ß-Gal Antibody

Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.

天然蛋白

No

No

酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫印迹

User Optimized

一抗

未共轭

$445.00

P00722

NP_414878.1

抗b半乳糖苷酶抗体-免疫印迹