抗体

p19Arf抗体

200-501-892

100微克

369美元

单克隆

大鼠

未共轭

12.A1.1

人类, 小鼠

免疫印迹, 酶联免疫吸附测定, 免疫沉淀

抗p19Arf(外显子1b)(大鼠)单克隆抗体-200-501

p19Arf抗体

This antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for western blotting reactivity should be optimized by the end user.  Expect a band approximately 19 kDa in size corresponding to mouse p19Arf by western blotting in the appropriate cell line or lysate.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

单克隆

12.A1.1

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:5, 000 - 1:20, 000

1:100

1:500 - 1:2, 000

Expiration date is one (1) year from date of opening.

IgG2b

Cdkn2a

大鼠

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This protein G purified monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 54-75 of mouse p19Arf protein.

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

This is a Protein G purified antibody directed against mouse p19Arf protein.  No reactivity is observed against human or hamster homologues.  The epitope was putatively mapped to amino acids 54-62 of mouse p19Arf.  BLAST analysis indicates that no significant sequence homology exists for this sequence with p19Arf homologues from other sources.   This epitope is also found on mouse p16Ink4a protein.  No additional information is available for reactivity with p19Arf protein from other sources.

人类, 小鼠, 仓鼠

Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

IgG2b

rat anti-p19Arf Antibody, Cyclin-dependent kinase inhibitor 2A isoform 3, p19 ARF

小鼠

The Ink4a-Arf locus encodes two tumor suppressor proteins, p16Ink4a and p19Arf.  Expression of Ink4a and Arf is regulated by distinct promoters upstream of alternative first exons whose products are spliced to a common second exon translated in alternative reading frames (from which Arf gets its name). The two genes are induced by different stress signals and can be separately mutated or silenced in tumor cells.  Targeted disruption of either or both genes in mouse leads to spontaneous tumor formation, which is accelerated by exposure of the mutant animals to chemical carcinogens or ionizing radiation.  Inactivation of ARF by mutation, deletion, or epigenetic silencing is observed in many human cancers, underscoring the role of this protein as a potent and ubiquitous tumor suppressor.  p19Arf  is activated by Myc or mutant Ras.  Once activated, p19Arf binds to the p53 negative regulator Mdm2, leading to p53 stabilization and unleashing a p53-dependent transcriptional program that triggers either cell cycle arrest or apoptosis.  p19Arf also has p53-independent inhibitory effects on cell proliferation.  p19Arf is a nucleolar protein and is implicated in ribosomal biogenesis.

Quelle,D.E., Zindy,F., Ashmun,R.A. and Sherr,C.J. (1995) Alternative reading frames of the INK4a tumor suppressor gene encode two unrelated proteins capable of inducing cell cycle arrest. Cell 83 (6), 993-1000. Serrano, M., Hannon, G. J. & Beach, D. (1993) A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. Nature 366, 704–707. Kamijo, T., Zindy, F., Roussel, M. F., Quelle, D. E., Downing, J. R., Ashmun, R. A., Grosveld, G. & Sherr, C. J. (1997) Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product p19ARF. Cell 91, 649–659.

Bertwistle D, Zindy F, Sherr CJ, Roussel MF. (2004) Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein. Hybrid Hybridomics 23(5):293-300. PMID: 15672607.

多肽

No

No

内部

酶联免疫吸附测定, 免疫沉淀, 免疫印迹

User Optimized

一抗

未共轭

369美元

Q64364

1162947

抗p19Arf抗体-免疫印迹