抗体

肿瘤坏死因子甲抗体

209-401-306-0100

100微升

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化

抗人类TNF-α(兔)抗体-209-401

肿瘤坏死因子甲抗体

This IgG fraction antibody of anti-Human TNFα has been tested for use in neutralizations, ELISA, immunohistochemistry and immunoblotting.  It recognizes the 17,000 MW TNFα.  Reactivity in other immunoassays is unknown.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

多克隆

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100微升

微升

1:1, 000 - 1:5, 000

1:100 - 1:500

User Optimized

1:500 - 1:2, 000

Expiration date is one (1) year from date of opening.

IgG

肿瘤坏死因子

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

TNF alpha antibody used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFα.

干冰

Liquid (sterile filtered)

This antibody is primarily directed against mature 17,000 MW human TNFα and is useful in determining its presence in various assays.  In general, this antibody also detects primate TNFα in the same formats using similar dilutions.  The antibody does not recognize human TNFß (lymphotoxin).   This IgG fraction antibody will recognize the cell-bound precursor of TNFα as a 26,000 protein in immunoblots, particularly in denatured samples.  This antibody is also useful for neutralization of human and primate TNFα activity in bioassays.  It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG is recommended.

人类

Store anti-TNF alpha at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, Tumour Necrosis Factor Alpha antibody, rabbit anti-Tumor Necrosis Factor Alpha Antibody, rabbit anti-TNF Alpha Antibody, Tumor necrosis factor, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2

人类

Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).

重组蛋白

No

No

酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫印迹

User Optimized

一抗

未共轭

P01375

P01375.1

抗人类TNFa抗体-免疫组化