公司名称 :
Rockland Immunochemicals
产品类型 :
产品名称 :
目录 :
规格 :
价格 :
克隆性 :
宿主 :
共轭标签 :
反应物种 :
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化
更多信息或购买 :
目录号 :
名称 :
显示名称 :
应用注释 :
This IgG fraction antibody of anti-Human TNFα has been tested for use in neutralizations, ELISA, immunohistochemistry and immunoblotting.  It recognizes the 17,000 MW TNFα.  Reactivity in other immunoassays is unknown.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
浓度值 :
浓度定义 :
by UV absorbance at 280 nm
共轭 :
规格 :
默认单元 :
ELISA稀释浓度 :
1:1, 000 - 1:5, 000
免疫组化稀释度 :
1:100 - 1:500
免疫荧光显微镜稀释浓度 :
User Optimized
免疫印迹稀释浓度 :
1:500 - 1:2, 000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
基因名称 :
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
TNF alpha antibody used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFα.
包装类型 :
物理状态 :
Liquid (sterile filtered)
纯度和特异性 :
This antibody is primarily directed against mature 17,000 MW human TNFα and is useful in determining its presence in various assays.  In general, this antibody also detects primate TNFα in the same formats using similar dilutions.  The antibody does not recognize human TNFß (lymphotoxin).   This IgG fraction antibody will recognize the cell-bound precursor of TNFα as a 26,000 protein in immunoblots, particularly in denatured samples.  This antibody is also useful for neutralization of human and primate TNFα activity in bioassays.  It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG is recommended.
物种活性 :
储存 :
Store anti-TNF alpha at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, Tumour Necrosis Factor Alpha antibody, rabbit anti-Tumor Necrosis Factor Alpha Antibody, rabbit anti-TNF Alpha Antibody, Tumor necrosis factor, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2
靶物种 :
背景 :
Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
一般参考 :
Cseh, K. and B. Beutler (1989) Alternative cleavage of the cachectin/tumor necrosis factor propeptide results in a larger, inactive form of secreted protein. J Biol Chem 264 (27): 16256-60.
免疫原类型 :
低内毒素 :
样品尺寸 :
应用文本 :
酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫印迹
其他 :
User Optimized
类别 :
共轭名称 :
2020价格 :
UniProt数据库 :
最初图像名称 :
更多信息或购买 :
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。