抗体

IL-6抗体

209-401-310

100微克

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化

抗人类IL-6(兔)抗体-209-401

IL-6抗体

This purified antibody has been tested for use in ELISA, IHC and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

多克隆

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:10,000

1:200 - 1:1, 000

1:300 - 1:1, 000

Expiration date is one (1) year from date of opening.

IgG

白细胞介素-6

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with mature length recombinant human IL-6 produced in E.coli.

Ambient

冻干

IL-6 antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.  The antiserum is also useful for neutralization of human of IL-6 activity in bioassays.  For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.  In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6.  However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. This product has minimal reactivity with mouse IL-6.

Restore with deionized water (or equivalent)

100微升

人类

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

兔抗IL-6抗体, 兔抗白细胞介素6抗体, HSF, 杂交瘤细胞生长因子, 杂交瘤细胞浆细胞瘤生长因子, IFNB2, 白细胞介素6, 白细胞介素-6蛋白, 干扰素β2, 白细胞介素6, BSF2, CDF

人类

Anti-IL-6 antibody is validated by ELISA, Immunohistochemistry and Western Blot Assays. IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

重组蛋白

No

Yes

No

酶联免疫吸附测定, 免疫组化, 免疫印迹

User Optimized

一抗

未共轭

P05231

NP_000591.1

抗人类白细胞介素-6抗体-免疫印迹