产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
血管内皮生长因子抗体过氧化物酶 共轭标签
目录 :
209-403-B99
规格 :
100微克
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
HRP
反应物种 :
人类
应用 :
免疫印迹, 免疫组化
更多信息或购买 :
产品信息
目录号 :
209-403-B99
名称 :
抗血管内皮生长因子-165(兔)抗体过氧化物酶 共轭标签-209-403
显示名称 :
血管内皮生长因子抗体过氧化物酶 共轭标签
应用注释 :
This protein-A purified antibody has been tested for use in western blotting. Reactivity in other assays has not been determined. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 22 kDa in size corresponding to monomeric human VEGF-165 protein by western blotting in the appropriate cell lysate or extract.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
1.0毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
HRP
规格 :
100微克
默认单元 :
微克
免疫组化稀释度 :
1:500-1:2,500
免疫印迹稀释浓度 :
1:1,000-1:5,000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG
基因名称 :
血管内皮生长因子A
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.
包装类型 :
Ambient
物理状态 :
冻干
防腐剂 :
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
纯度和特异性 :
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human VEGF-165 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
重组缓冲液 :
Restore with deionized water (or equivalent)
重组体积 :
100微升
物种活性 :
人类
稳定剂 :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
储存 :
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
rabbit anti-VEGF Peroxidase Conjugated antibody, rabbit anti-VEGF-165 Peroxidase Conjugated antibody, Vascular endothelial growth factor A, VEGF-A, VEGF-165, VEGF165, VEGF isoform L, Vascular permeability factor, VPF
靶物种 :
人类
背景 :
VEGF (Vascular Endothelial Growth Factor A) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. The sequence of this isoform differs from the canonical sequence as follows: 141-141: K → N and 142-182: missing. This isoform is often found as a disulfide linked homodimer.
免疫原类型 :
重组蛋白
低内毒素 :
No
样品尺寸 :
No
应用文本 :
免疫组化, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
Peroxidase (Horseradish)
UniProt数据库 :
P15692
NCBI :
NP_001165097.1
最初图像名称 :
过氧化物酶 -免疫印迹
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。