血球凝集素表位标签抗体
Rockland Immunochemicals
目录: 600-401-384
domestic rabbit 多克隆
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 染色质免疫沉淀
文章摘录数: 45

Rockland's Affinity Purified anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by IHC on formalin fixed paraffin embedded tissue. Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 µm were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2,000 and reacted at 4° C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayer's hematoxylin. Personal Communication, Behzad Yeganeh,U. Manitoba, Winnipeg, Canada.
规格: 100
价格: https://www.rockland.com/search/?searchString=600-401-384
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血球凝集素表位标签抗体生物素共轭标签
Rockland Immunochemicals
目录: 600-406-384
domestic rabbit 多克隆
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化

Anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by western blot. Polyclonal rabbit-anti-HA epitope tag at a 1:10,000 dilution was used to detect 1.0 ug of recombinant transcription factor protein containing the HA epitope tag. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with Rockland's anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:4000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results
规格: 100微克
价格:
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血球凝集素表位标签抗体过氧化物酶 共轭标签
Rockland Immunochemicals
目录: 600-403-384
domestic rabbit 多克隆
共轭标签: HRP
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
规格: 100微克
价格:
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HA Epitope Tag Antibody Dylight™ 488 Conjugated
Rockland Immunochemicals
目录: 600-441-384
domestic rabbit 多克隆
共轭标签: DyLight488
应用: 免疫印迹

Properties of Dylight™ Conjugates.

Western Blot of Rabbit anti-HA DyLight™ 488 Conjugated Antibody. Lane 1: 12 Epitope Tag Protein Marker Lysate. Lane 2: None. Load: 10 uL. Primary antibody: None. Secondary antibody: DyLight™ 488 rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 69 kDa, 69 kDa for HA. Other band(s): None.
规格: 100微克
价格:
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HA Epitope Tag Antibody Dylight™ 549 Conjugated
Rockland Immunochemicals
目录: 600-442-384
domestic rabbit 多克隆
共轭标签: DyLight549
应用: 免疫印迹


规格: 100微克
价格:
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HA Epitope Tag Antibody Dylight™ 649 Conjugated
Rockland Immunochemicals
目录: 600-443-384
domestic rabbit 多克隆
共轭标签: DyLight649
应用: 免疫印迹

DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
规格: 100微克
价格:
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HA Epitope Tag Antibody Dylight™ 680 Conjugated
Rockland Immunochemicals
目录: 600-444-384
domestic rabbit 多克隆
共轭标签: DyLight680
应用: 免疫印迹

规格: 100微克
价格:
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HA Epitope Tag Antibody Dylight™ 800 Conjugated
Rockland Immunochemicals
目录: 600-445-384
domestic rabbit 多克隆
共轭标签: DyLight800
应用: 免疫印迹

规格: 100微克
价格:
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HA Epitope Tag Antibody Dylight™ 405 Conjugated
Rockland Immunochemicals
目录: 600-446-384
domestic rabbit 多克隆
共轭标签: DyLight系列染料405
应用: 免疫印迹

Properties of DyLight™ Fluorescent Dyes.
规格: 100微克
价格:
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