domestic rabbit 多克隆
反应物种: 人类, 小鼠, 牛
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-冰冻切片
文章摘录数: 136
反应物种: 人类, 小鼠, 牛
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-冰冻切片
文章摘录数: 136
Western blot analysis is shown using Rockland's Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 µg) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2–2 µg/10 ml in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% Non-fat milk. Detection occurred by incubation with a horseradish peroxidase-conjugated secondary antibody at 1 µg/10 ml. Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences). Other detection systems will yield similar results. See Hazra et al. (2004) for additional details.
Western blot of Human Collagen Type I. Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.
规格: 100
价格: 459.00美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠, 牛
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 流式细胞仪
文章摘录数: 120
反应物种: 人类, 小鼠, 大鼠, 牛
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 流式细胞仪
文章摘录数: 120
Rockland's Affinity Purified anti-Collagen I antibody was used at a 1:100 dilution to detect distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. The antibody was reacted with antibody for 4 hours at room temperature followed by the addition of secondary antibody and substrate reaction. Tissue was formalin-fixed and paraffin embedded. No antigen retrieval was performed.
规格: 500
价格: https://www.rockland.com/search/?searchString=600-401-103-0.5
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠, 牛
共轭标签: 异硫氰酸荧光素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 流式细胞仪
文章摘录数: 2
反应物种: 人类, 小鼠, 大鼠, 牛
共轭标签: 异硫氰酸荧光素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 流式细胞仪
文章摘录数: 2
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠, 牛
共轭标签: HRP
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 流式细胞仪
反应物种: 人类, 小鼠, 大鼠, 牛
共轭标签: HRP
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 流式细胞仪