domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 流式细胞仪, 抑制或激活实验
文章摘录数: 2
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 流式细胞仪, 抑制或激活实验
文章摘录数: 2
IL-1B was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1B by Western blot. Lane 1 and 2 were loaded with 2.5 ?g and 500 ng of dog IL-1B respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3,000 dilution of IRDye™800 conjugated Donkey anti-Rabbit IgG (code # 611-732-127) for 1h at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
规格: 1
价格: https://www.rockland.com/search/?searchString=209-401-301
至产商
domestic rabbit 多克隆
反应物种: 人类, 犬
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀
反应物种: 人类, 犬
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀
domestic rabbit 多克隆
反应物种: 人类, 犬
应用: 免疫印迹, 酶联免疫吸附测定
反应物种: 人类, 犬
应用: 免疫印迹, 酶联免疫吸附测定
Western blot showing detection of recombinant human IL-1ß by Rockland's anti-IL-1ß antibody. Recombinant human IL-1ß was loaded on to an SDS-PAGE gel at 0.1 µg and after separation, transferred to nitrocellulose. The expected band is approximately 18 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
规格: 100微克
价格:
至产商
domestic rabbit 多克隆
反应物种: 人类, 犬
共轭标签: HRP
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
反应物种: 人类, 犬
共轭标签: HRP
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
domestic rabbit 多克隆
反应物种: 人类, 犬
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
反应物种: 人类, 犬
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化