产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
其他
产品名称 :
Recombinant Anti-TNF alpha Fab Antibody
目录 :
400-001-MT3
规格 :
50
价格 :
465.00美元
更多信息或购买 :
图像
图像 1 :
Western Blot using Adalimumab, Anti-human TNF alpha Fab fragment to detect human TNA alpha. Lanes 1, 3, and 5: TNF alpha reduced. Lanes 2, 4, 6: TNF alpha non-reduced. MW: Molecular Weight Ladder. Protein Load: Lane 1-2: 200ng. Lane 3-4: 40ng. Lane 5-6: 8ng. Primary Antibody: Anti-TNF alpha Fab fragment Antibody at 1.0µg/mL for 2 hours at 4°C. Secondary Antibody: F(ab')2 Human IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed (p/n 709-1318) at 1:5,000 dilution was used. The protein has a calculated MW of 18.3kDa. The protein migrates as 18.2kDa under reducing (R) and non-reducing condition (SDS-PAGE).
产品信息
目录号 :
400-001-MT3
名称 :
Humanized Recombinant Anti-Human TNFα Fab fragment Antibody - 400-001-MT3
显示名称 :
Recombinant Anti-TNF alpha Fab Antibody
应用注释 :
Humanized Recombinant Anti-Anti-TNFα Fab fragment Antibody has been tested for use in Western Blot and ELISA. This antibody recognizes structured TNFα and will work in western blot when the protein has not been denatured with DTT or bMe. Although not tested, this antibody could be useful in in IHC and in in-vivo and other cellular assays. Specific conditions for reactivity should be optimized by the end user.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
重组单克隆
克隆编号 :
Abz520
浓度值 :
0.42毫克/毫升
共轭 :
(None)
规格 :
50
默认单元 :
微克
ELISA稀释浓度 :
User Optimized
免疫印迹稀释浓度 :
User Optimized
有效期 :
Expiration date is six (6) months from date of opening.
格式 :
IgG Fab
基因名称 :
肿瘤坏死因子甲
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
纯度和特异性 :
Humanized Recombinant TNFα Fab fragment was expressed in CHO cells. The purity was estimated to be >95% by SDS-PAGE analysis.
物种活性 :
H. sapiens (Human)
检测过的应用 :
酶联免疫吸附测定;免疫印迹
稳定剂 :
30% 甘油
储存 :
Store vial at -20° C. Dilute only prior to immediate use.
子种类 :
IgG1
同义词 :
APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, Tumour Necrosis Factor Alpha antibody, rabbit anti-Tumor Necrosis Factor Alpha Antibody, rabbit anti-TNF Alpha Antibody, Tumor necrosis factor, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2
靶物种 :
人类
背景 :
Anti-TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNF is an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD). Anti-TNF alpha antibody, Adalimumab, a monoclonal antibody, working by inactivating TNFα is approved to treat rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, Crohn's disease, ulcerative colitis, psoriasis, hidradenitis suppurativa, uveitis, and juvenile idiopathic arthritis and sold under the brand name Humira. This antibody is made from a Adalimumab Fab fragment making it specific, efficient, and effective. Humanized Recombinant Anti-TNFα Fab fragment Antibody is useful for researchers interested in Cancer and Inflammatory disease research.
免疫原类型 :
重组蛋白
低内毒素 :
No
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, 免疫印迹
类别 :
一抗
共轭名称 :
未共轭
2023价格 :
465.00美元
UniProt数据库 :
P01375
NCBI :
7124
最初图像名称 :
Western Blot of Anti-human TNF alpha Fab fragment Antibody
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部，为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立，利用In vitro及In vivo方法制备并纯化单抗及多抗，这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究：Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。