抗体

Recombinant scFv Anti-Human CD45 Antibody

400-301-MR2

100微克

重组

小鼠

未共轭

BC8

酶联免疫吸附测定, 流式细胞仪

Recombinant scFv fragment Anti-Human CD45 (Mouse) Antibody - 400-301-MR2

Recombinant scFv Anti-Human CD45 Antibody

Recombinant scFv fragment Anti-Human CD45 Monoclonal Antibody has been tested in ELISA Capture, Flow Cytometry, Immunofluorescence. This antibody has been diluted for recommended use per test. Typically, we use 1×10^6 cells in a 100μL experimental sample (a test). Optimal titers for applications should be determined by the researcher.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

重组单克隆

BC8

0.71毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

User Optimized

User Optimized

User Optimized

Expiration date is eighteen (18) months from date of opening.

IgG1

蛋白酪氨酸磷酸酶受体C

小鼠

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Human Peripheral Blood Leukocytes

干冰

Liquid (sterile filtered)

0.09%(w/v)叠氮化钠

The scFv antibody fragment was purified from disrupted Escherichia coli culture by column chromatography.

人类白细胞

Store undiluted at -20° C prior to opening. Protect from prolonged exposure to light.

IgG1

PTPRD;CD45R;LCA;L-CA;白细胞共同抗原

人类

CD45 is encoded by the PTPRC (Protein tyrosine phosphatase receptor type C) gene. CD45, also known as the leukocyte common antigen (LCA), is a member of the protein tyrosine phosphatase (PTP) family. It is present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and thymocytes. CD45 is absent from circulating erythrocytes, platelets, or mature erythroid cells of bone marrow and non-hemopoietic tissues. This is an anti-cd45 scFv antibody fragment. This scFv fragment representing the functional antigen binding fragment of immunoglobulins is produced in bacterial expression systems. Using biochemical procedures and/or genetic modification (recombinant DNA technology), these fragments can be coupled to compounds and proteins that allow for the detection of these fragments. Examples of such compounds include fluorophores (e.g. fluorescein) and enzymes (e.g. alkaline phosphatase) generating fluorescence. The resultant compounds now have bifunctional properties in that they can bind to their target via the scFv fragment and can be detected via their fluorescence marker. In addition to labeling the scFv with fluorophore markers, the scFv antibody fragment has been coupled to magnetic microparticles allowing for the capture of the scFv target complex in a magnetic field. An scFv fragment retains the complete antigen-binding capability of the whole monoclonal antibody, thus facilitating a unique molecule with the property of being used as either a diagnostic or therapeutic reagent. In addition, the scFv with exquisite specificity and affinity towards a specific target can also be used as a delivery vehicle for radioimmunoimaging and radioimmunotherapy. In addition, the small antigen-binding scFv offer several advantages over a whole antibody molecule in that the smaller size of the scFv allow it to penetrate more rapidly and evenly to tumors and can penetrate the blood brain-barrier. scFv fragments have more rapid clearance from blood, and therefore be coupled with drugs and radionuclides in order to result in low exposure of the healthy tissue. Also, there appears to be no uptake of the scFv by the kidney allowing them to efficiently localize to the tumors, an important property in cancer therapy.

天然蛋白

No

No

酶联免疫吸附测定, 流式细胞仪, IF显微镜

一抗

未共轭

P08575

NP_002829.3

Immunofluorescence of scFv fragment Anti-CD45 Antibody

Yes