抗体

VDAC/孔蛋白抗体

600-101-HB2

100

425.00美元

多克隆

domestic goat

未共轭

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化

抗VDAC/孔蛋白(山羊)抗体-600-101

VDAC/孔蛋白抗体

VDAC/Porin Antibody has been tested for use in ELISA, western blot, and Immunofluorescence.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30-33 kDa in size corresponding to VDAC/Porin by western blotting in the appropriate cell lysate or extract. Positive control used in WB: HeLa Whole Cell, Mouse Heart Lysate, or Rat Brain Tissue Lysate, and HeLa Cells in IF.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

多克隆

0.92毫克/毫升

by UV absorbance at 280 nm

(None)

100

微克

1:10,000-1:50,000

User Optimized

5微克/毫升

1微克/毫升

Expiration date is one (1) year from date of opening.

IgG

VDAC1

山羊

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

VDAC/Porin Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to N-terminal portion of Human VDAC1/Porin1.

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

Anti-VDAC/Porin Antibody is directed against human VDAC1/Porin1 protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 including mouse, rat, bovine, pig, and rabbit sources based on 100% homology for the immunogen sequence. Such broad reactivity makes this antibody useful as an excellent loading control.

H. sapiens (Human); Mus musculus (Mouse); Rattus (Rat)

酶联免疫吸附测定;IF;免疫印迹

Store VDAC antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Goat anti-VDAC/Porin antibody, VDAC-1 Loading Control Antibody, Porin Loading Control Antibody, VDAC-1 Antibody, VDAC1 Antibody, POR1 Antibody, Porin Antibody, Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL

人类

VDAC/Porin Antibody recognizes VDAC (also known as Voltage-dependent anion-selective channel protein 1) which is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger).  The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane.  VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis.  VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.  Diseases associated with VDAC1 include Cystic Fibrosis and Gerstmann-Straussler Disease. This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations. Anti-VDAC/Porin Antibody is useful for researchers interested in Apoptosis, cell survival and regulation, Mitochondrial Proteins, and Neuroscience Research.

多肽

No

No

N末端

酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫印迹

一抗

未共轭

425.00美元

P21796

7416

Immunofluorescence of Goat Anti-VDAC/Porin Antibody