产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
I型胶原抗体
目录 :
600-401-103-0.1
规格 :
100
价格 :
407.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠,
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 31
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 图 2a
Wu X, Dao Thi V, Huang Y, Billerbeck E, Saha D, Hoffmann H, et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 2018;172:423-438.e25 pubmed 出版商
  • 免疫组化-冰冻切片; 小鼠; 1:100; 图 2a
Guiraud S, Migeon T, Ferry A, Chen Z, Ouchelouche S, Verpont M, et al. HANAC Col4a1 Mutation in Mice Leads to Skeletal Muscle Alterations due to a Primary Vascular Defect. Am J Pathol. 2017;187:505-516 pubmed 出版商
  • 免疫组化; 小鼠; 1:500
Yang Z, Peng Y, Gopalan A, Gao D, Chen Y, Joyner A. Stromal hedgehog signaling maintains smooth muscle and hampers micro-invasive prostate cancer. Dis Model Mech. 2017;10:39-52 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:50; 图 2a
Hofmann K, Fiedler S, Vierkotten S, Weber J, Klee S, Jia J, et al. Classical transient receptor potential 6 (TRPC6) channels support myofibroblast differentiation and development of experimental pulmonary fibrosis. Biochim Biophys Acta Mol Basis Dis. 2017;1863:560-568 pubmed 出版商
  • 免疫组化-冰冻切片; 人类; 1:1000; 图 5d
Formica F, Öztürk E, Hess S, Stark W, Maniura Weber K, Rottmar M, et al. A Bioinspired Ultraporous Nanofiber-Hydrogel Mimic of the Cartilage Extracellular Matrix. Adv Healthc Mater. 2016;5:3129-3138 pubmed 出版商
  • 免疫印迹; 人类; 图 4
Zhang Y, Stefanovic B. Akt mediated phosphorylation of LARP6; critical step in biosynthesis of type I collagen. Sci Rep. 2016;6:22597 pubmed 出版商
Ida Y, Hikage F, Itoh K, Ida H, Ohguro H. Prostaglandin F2α agonist-induced suppression of 3T3-L1 cell adipogenesis affects spatial formation of extra-cellular matrix. Sci Rep. 2020;10:7958 pubmed 出版商
Wu Y, An C, Jin X, Hu Z, Wang Y. Disruption of CXCR6 Ameliorates Kidney Inflammation and Fibrosis in Deoxycorticosterone Acetate/Salt Hypertension. Sci Rep. 2020;10:133 pubmed 出版商
Chung S, Overstreet J, Li Y, Wang Y, Niu A, Wang S, et al. TGF-β promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration. JCI Insight. 2018;3: pubmed 出版商
Jager M, Lee M, Li C, Farmer S, Fried S, Layne M. Aortic carboxypeptidase-like protein enhances adipose tissue stromal progenitor differentiation into myofibroblasts and is upregulated in fibrotic white adipose tissue. PLoS ONE. 2018;13:e0197777 pubmed 出版商
Li Y, Chung S, Li Z, Overstreet J, Gagnon L, Grouix B, et al. Fatty acid receptor modulator PBI-4050 inhibits kidney fibrosis and improves glycemic control. JCI Insight. 2018;3: pubmed 出版商
Ho L, Skiba N, Ullmer C, Rao P. Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells. Invest Ophthalmol Vis Sci. 2018;59:1969-1984 pubmed 出版商
Murray L, Habiel D, Hohmann M, Camelo A, Shang H, Zhou Y, et al. Antifibrotic role of vascular endothelial growth factor in pulmonary fibrosis. JCI Insight. 2017;2: pubmed 出版商
Roselló Díez A, Stephen D, Joyner A. Altered paracrine signaling from the injured knee joint impairs postnatal long bone growth. elife. 2017;6: pubmed 出版商
He X, Bougioukli S, Ortega B, Arevalo E, Lieberman J, McMahon A. Sox9 positive periosteal cells in fracture repair of the adult mammalian long bone. Bone. 2017;103:12-19 pubmed 出版商
Yosef R, Pilpel N, Papismadov N, Gal H, Ovadya Y, Vadai E, et al. p21 maintains senescent cell viability under persistent DNA damage response by restraining JNK and caspase signaling. EMBO J. 2017;36:2280-2295 pubmed 出版商
Surolia R, Li F, Wang Z, Li H, Liu G, Zhou Y, et al. 3D pulmospheres serve as a personalized and predictive multicellular model for assessment of antifibrotic drugs. JCI Insight. 2017;2:e91377 pubmed 出版商
Pereira C, Teixeira G, Ribeiro Machado C, Caldeira J, Costa M, Figueiredo F, et al. Mesenchymal Stem/Stromal Cells seeded on cartilaginous endplates promote Intervertebral Disc Regeneration through Extracellular Matrix Remodeling. Sci Rep. 2016;6:33836 pubmed 出版商
Komatsu Y, Ibi M, Chosa N, Kyakumoto S, Kamo M, Shibata T, et al. Zoledronic acid suppresses transforming growth factor-?-induced fibrogenesis by human gingival fibroblasts. Int J Mol Med. 2016;38:139-47 pubmed 出版商
Clevenger T, Hinman C, Ashley Rubin R, Smither K, Burke D, Hawker C, et al. Vitronectin-Based, Biomimetic Encapsulating Hydrogel Scaffolds Support Adipogenesis of Adipose Stem Cells. Tissue Eng Part A. 2016;22:597-609 pubmed 出版商
Huang H, Wang S, Gui J, Shen H. A study to identify and characterize the stem/progenitor cell in rabbit meniscus. Cytotechnology. 2016;68:2083-103 pubmed 出版商
Chen P, Qin L, Baeyens N, Li G, Afolabi T, Budatha M, et al. Endothelial-to-mesenchymal transition drives atherosclerosis progression. J Clin Invest. 2015;125:4514-28 pubmed 出版商
O Flaherty B, Matar C, Wakeman B, Garcia A, Wilke C, Courtney C, et al. CD8+ T Cell Response to Gammaherpesvirus Infection Mediates Inflammation and Fibrosis in Interferon Gamma Receptor-Deficient Mice. PLoS ONE. 2015;10:e0135719 pubmed 出版商
Zwolanek D, Flicker M, Kirstätter E, Zaucke F, van Osch G, Erben R. β1 Integrins Mediate Attachment of Mesenchymal Stem Cells to Cartilage Lesions. Biores Open Access. 2015;4:39-53 pubmed 出版商
Guiraud S, Squire S, Edwards B, Chen H, Burns D, Shah N, et al. Second-generation compound for the modulation of utrophin in the therapy of DMD. Hum Mol Genet. 2015;24:4212-24 pubmed 出版商
Luo Y, Xu W, Chen H, Warburton D, Dong R, Qian B, et al. A novel profibrotic mechanism mediated by TGFβ-stimulated collagen prolyl hydroxylase expression in fibrotic lung mesenchymal cells. J Pathol. 2015;236:384-94 pubmed 出版商
Mak K, Kwong A, Chu E, Hoo N. Hepatic steatosis, fibrosis, and cancer in elderly cadavers. Anat Rec (Hoboken). 2012;295:40-50 pubmed 出版商
Crawford J, Pilling D, Gomer R. Improved serum-free culture conditions for spleen-derived murine fibrocytes. J Immunol Methods. 2010;363:9-20 pubmed 出版商
She H, Xiong S, Hazra S, Tsukamoto H. Adipogenic transcriptional regulation of hepatic stellate cells. J Biol Chem. 2005;280:4959-67 pubmed
Hazra S, Xiong S, Wang J, Rippe R, Krishna V, Chatterjee K, et al. Peroxisome proliferator-activated receptor gamma induces a phenotypic switch from activated to quiescent hepatic stellate cells. J Biol Chem. 2004;279:11392-401 pubmed
Stefanovic B, Schnabl B, Brenner D. Inhibition of collagen alpha 1(I) expression by the 5' stem-loop as a molecular decoy. J Biol Chem. 2002;277:18229-37 pubmed
产品信息
目录号 :
600-401-103-0.1
名称 :
抗I型胶原(兔)抗体-600-401.1
显示名称 :
I型胶原抗体
应用注释 :
Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, Immunofluorescence, FLOW, and western blotting for highly sensitive qualitative analysis.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
1.08毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100
默认单元 :
微克
ELISA稀释浓度 :
1:5, 000 - 1:50, 000
FLISA稀释浓度 :
1:100
流式细胞仪稀释浓度 :
User Optimized
免疫组化稀释度 :
1:50 - 1:200
免疫荧光显微镜稀释浓度 :
User Optimized
免疫沉淀稀释度 :
1:100
免疫印迹稀释浓度 :
1:1, 000 - 1:10, 000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG
基因 :
COL1A1/A2
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
I型胶原来自人类和牛胎盘
包装类型 :
Wet Ice
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.  Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.   This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens.  Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
物种活性 :
Bovine (Bos Taurus); H. sapiens (Human)
储存 :
Store vial at 4° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
同义词 :
rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1
靶物种 :
Mammalian
背景 :
Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.
免疫原类型 :
天然蛋白
低内毒素 :
No
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, FLISA, 流式细胞仪, 免疫组化, IF显微镜, 免疫沉淀, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2022价格 :
407.00美元
UniProt数据库 :
P02452
NCBI :
P02452.5
最初图像名称 :
抗胶原I抗体-免疫组化
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。