产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
绿色荧光蛋白抗体
目录 :
600-401-215
规格 :
100
价格 :
https://www.rockland.com/search/?searchString=600-401-215
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 流式细胞仪
更多信息或购买 :
Rockland Immunochemicals 产品网页
文章摘录数: 62
参考文献
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Petrov K, Wierbowski B, Liu J, Salic A. Distinct Cation Gradients Power Cholesterol Transport at Different Key Points in the Hedgehog Signaling Pathway. Dev Cell. 2020;55:314-327.e7 pubmed 出版商
Antón Z, Mullally G, Ford H, van der Kamp M, Szczelkun M, Lane J. Mitochondrial import, health and mtDNA copy number variability seen when using type II and type V CRISPR effectors. J Cell Sci. 2020;133: pubmed 出版商
Jiwani T, Kim J, Rosenblum N. Suppressor of fused controls cerebellum granule cell proliferation by suppressing Fgf8 and spatially regulating Gli proteins. Development. 2020;147: pubmed 出版商
Wei J, Long L, Zheng W, Dhungana Y, Lim S, Guy C, et al. Targeting REGNASE-1 programs long-lived effector T cells for cancer therapy. Nature. 2019;576:471-476 pubmed 出版商
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Zhang Z, Sliter D, Bleck C, Ding S. Fis1 deficiencies differentially affect mitochondrial quality in skeletal muscle. Mitochondrion. 2019;49:217-226 pubmed 出版商
Choi S, Choi E, Cho Y, Nam D, Lee J, Lee E. The Salmonella virulence protein MgtC promotes phosphate uptake inside macrophages. Nat Commun. 2019;10:3326 pubmed 出版商
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Ferrari M, Malardé V, Grassart A, Salavessa L, Nigro G, Descorps Declère S, et al. Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport. Proc Natl Acad Sci U S A. 2019;: pubmed 出版商
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Jo S, Fonseca T, Bocco B, Fernandes G, McAninch E, Bolin A, et al. Type 2 deiodinase polymorphism causes ER stress and hypothyroidism in the brain. J Clin Invest. 2019;129:230-245 pubmed 出版商
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Schwarz B, Hollfelder D, Scharf K, Hartmann L, Reim I. Diversification of heart progenitor cells by EGF signaling and differential modulation of ETS protein activity. elife. 2018;7: pubmed 出版商
Bao X, Spanos C, Kojidani T, Lynch E, Rappsilber J, Hiraoka Y, et al. Exportin Crm1 is repurposed as a docking protein to generate microtubule organizing centers at the nuclear pore. elife. 2018;7: pubmed 出版商
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产品信息
目录号 :
600-401-215
名称 :
抗绿色荧光蛋白(兔)抗体-600-401
显示名称 :
绿色荧光蛋白抗体
应用注释 :
Anti-GFP antibody is designed to detect GFP and its variants. GFP antibody has been tested by western blot and ELISA. This product can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
1.25毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100
默认单元 :
微克
ELISA稀释浓度 :
1:20, 000 - 1:120, 000
流式细胞仪稀释浓度 :
User Optimized
免疫组化稀释度 :
1:200 - 1:3, 000
免疫荧光显微镜稀释浓度 :
1:500 - 1:5, 000
免疫沉淀稀释度 :
User Optimized
免疫印迹稀释浓度 :
1:500 - 1:5, 000
有效期 :
Expiration date is one (1) year from date of receipt.
格式 :
IgG
宿主动物 :
兔
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
其他性能数据 :
Immuno-EM; TEM
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).  No reaction was observed against Human, Mouse or Rat serum proteins.
物种活性 :
绿色荧光蛋白;eGFP;rGFP;RS-绿色荧光蛋白;S65T-绿色荧光蛋白;YFP
检测过的应用 :
酶联免疫吸附测定;免疫印迹
Suggested Applications :
EM; IF; IHC; IP; Multiplex; Other; Purification
储存 :
Store Anti-GFP Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. GFP antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
背景 :
Green Fluorescent Protein (GFP) is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP Antibody is ideal for Cell Biology, Neuroscience and Cancer research.
免疫原类型 :
重组蛋白
低内毒素 :
No
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, 流式细胞仪, 免疫组化, IF显微镜, 免疫沉淀, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2024价格 :
$339.00
UniProt数据库 :
P42212
最初图像名称 :
Anti GFP antibody - Immunomicroscopy
更多信息或购买 :
Rockland Immunochemicals 产品网页
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部，为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立，利用In vitro及In vivo方法制备并纯化单抗及多抗，这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究：Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。