抗体

磷酸酪氨酸抗体

600-401-262

100微克

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀

Anti-PhosphoTyrosine (pY) (RABBIT) Antibody - 600-401-262

磷酸酪氨酸抗体

Anti-PhosphoTyrosine (pY) Antibody is suitable for use in ELISA, western blotting and immunoprecipitation assays.  Anti-PhosphoTyrosine (pY) Antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphothreonine or phosphoserine residues. Anti-PhosphoTyrosine (pY) Antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, Anti-PhosphoTyrosine (pY) Antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

多克隆

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:1, 000- 1:5, 000

1:50

10.0微克/500微克样本

1:500

Expiration date is one (1) year from date of opening.

IgG

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This Anti-PhosphoTyrosine antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

Anti-PhosphoTyrosine (pY) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated tyrosine peptide coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.  Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphothreonine or phosphoserine.

磷酸酪氨酸

Store PhosphoTyrosine antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

PhosphoTyrosine (pY)reactive antibody, PhosphoTyrosine (pY) polyclonal antibody, PhosphoTyrosine antibody produced in rabbit

Phosphotyrosine is the tyrosine amino acid that has been phosphorylated and is considered one of the integral steps in signal transduction and general enzyme activity. Tyrosine is phosphorylated through protein kinases and through phosphorylation, phosphotyrosine can bind other proteins causing binding of multi-protein complexes that connect the upstream kinases and downstream effectors. Phosphotyrosine-binding domains, or PTB's, are pervasive throughout the study of signal transduction and disease. Anti-Phosphotyrosine (pY) Antibody is ideal for investigators in Cancer, Signal Transduction, and Cell Signaling research.

其他

No

No

酶联免疫吸附测定, 免疫组化, 免疫沉淀, 免疫印迹

User Optimized

一抗

未共轭