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文章摘录数: 169
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Hannafon B, Gin A, Xu Y, Bruns M, Calloway C, Ding W. Metastasis-associated protein 1 (MTA1) is transferred by exosomes and contributes to the regulation of hypoxia and estrogen signaling in breast cancer cells. Cell Commun Signal. 2019;17:13 pubmed 出版商
Narbonne Reveau K, Maurange C. Developmental regulation of regenerative potential in Drosophila by ecdysone through a bistable loop of ZBTB transcription factors. PLoS Biol. 2019;17:e3000149 pubmed 出版商
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Murakami Y, Naganuma H, Tanigawa S, Fujimori T, Eto M, Nishinakamura R. Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation. Sci Rep. 2019;9:1172 pubmed 出版商
Spence E, Dube S, Uezu A, Locke M, Soderblom E, Soderling S. In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation. Nat Commun. 2019;10:386 pubmed 出版商
Tang Y, Yang G, Zhang J, Li X, Zhang C, Wang Y, et al. E-cadherin is Required for the Homeostasis of Lgr5+ Gastric Antral Stem Cells. Int J Biol Sci. 2019;15:34-43 pubmed 出版商
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Panayotis N, Sheinin A, Dagan S, Tsoory M, Rother F, Vadhvani M, et al. Importin α5 Regulates Anxiety through MeCP2 and Sphingosine Kinase 1. Cell Rep. 2018;25:3169-3179.e7 pubmed 出版商
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Ahmed S, Nishida Fukuda H, Li Y, McDonald W, Gradinaru C, Macara I. Exocyst dynamics during vesicle tethering and fusion. Nat Commun. 2018;9:5140 pubmed 出版商
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Janssen A, Katrukha E, van Straaten W, Verlhac P, Reggiori F, Kapitein L. Probing aggrephagy using chemically-induced protein aggregates. Nat Commun. 2018;9:4245 pubmed 出版商
Jaeger A, Stanley M, Weiss Z, Musso P, Chan R, Zhang H, et al. A complex peripheral code for salt taste in Drosophila. elife. 2018;7: pubmed 出版商
Wang J, Batourina E, Schneider K, Souza S, Swayne T, Liu C, et al. Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication. Cell Rep. 2018;25:464-477.e4 pubmed 出版商
Rossidis A, Stratigis J, Chadwick A, Hartman H, Ahn N, Li H, et al. In utero CRISPR-mediated therapeutic editing of metabolic genes. Nat Med. 2018;24:1513-1518 pubmed 出版商
Smith C, Frolinger T, Brathwaite J, Sims S, Pasinetti G. Dietary polyphenols enhance optogenetic recall of fear memory in hippocampal dentate gyrus granule neuron subpopulations. Commun Biol. 2018;1:42 pubmed 出版商
Lu Y, Cao J, Napoli M, Xia Z, Zhao N, Creighton C, et al. miR-205 Regulates Basal Cell Identity and Stem Cell Regenerative Potential During Mammary Reconstitution. Stem Cells. 2018;36:1875-1889 pubmed 出版商
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Johnson B, Coutts M, Vo H, Hao X, Fatima N, Rivera M, et al. Accurate, strong, and stable reporting of choroid plexus epithelial cells in transgenic mice using a human transthyretin BAC. Fluids Barriers CNS. 2018;15:22 pubmed 出版商
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Moehlman A, Casey A, Servage K, Orth K, KRAMER H. Adaptation to constant light requires Fic-mediated AMPylation of BiP to protect against reversible photoreceptor degeneration. elife. 2018;7: pubmed 出版商
Roselló Díez A, Madisen L, Bastide S, Zeng H, Joyner A. Cell-nonautonomous local and systemic responses to cell arrest enable long-bone catch-up growth in developing mice. PLoS Biol. 2018;16:e2005086 pubmed 出版商
Ye W, Takabayashi H, Yang Y, Mao M, Hibdon E, Samuelson L, et al. Regulation of Gastric Lgr5+ve Cell Homeostasis by Bone Morphogenetic Protein (BMP) Signaling and Inflammatory Stimuli. Cell Mol Gastroenterol Hepatol. 2018;5:523-538 pubmed 出版商
Cao Y, Wang H, Wang Q, Han X, Zeng W. Three-dimensional volume fluorescence-imaging of vascular plasticity in adipose tissues. Mol Metab. 2018;14:71-81 pubmed 出版商
Rowland M, Jiang Y, Beier F, Berube N. Inactivation of hepatic ATRX in Atrx Foxg1cre mice prevents reversal of aging-like phenotypes by thyroxine. Aging (Albany NY). 2018;10:1223-1238 pubmed 出版商
Suzuki I, Gacquer D, Van Heurck R, Kumar D, Wojno M, Bilheu A, et al. Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through Delta/Notch Regulation. Cell. 2018;173:1370-1384.e16 pubmed 出版商
Ting J, Kalmbach B, Chong P, de Frates R, Keene C, Gwinn R, et al. A robust ex vivo experimental platform for molecular-genetic dissection of adult human neocortical cell types and circuits. Sci Rep. 2018;8:8407 pubmed 出版商
Stuhlmann T, Planells Cases R, Jentsch T. LRRC8/VRAC anion channels enhance β-cell glucose sensing and insulin secretion. Nat Commun. 2018;9:1974 pubmed 出版商
Chung M, Bujnis M, Barkauskas C, Kobayashi Y, Hogan B. Niche-mediated BMP/SMAD signaling regulates lung alveolar stem cell proliferation and differentiation. Development. 2018;145: pubmed 出版商
Diekman B, Sessions G, Collins J, Knecht A, Strum S, Mitin N, et al. Expression of p16INK4a is a biomarker of chondrocyte aging but does not cause osteoarthritis. Aging Cell. 2018;17:e12771 pubmed 出版商
Ueno M, Nakamura Y, Li J, Gu Z, Niehaus J, Maezawa M, et al. Corticospinal Circuits from the Sensory and Motor Cortices Differentially Regulate Skilled Movements through Distinct Spinal Interneurons. Cell Rep. 2018;23:1286-1300.e7 pubmed 出版商
Dhandapani R, Arokiaraj C, Taberner F, Pacifico P, Raja S, Nocchi L, et al. Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons. Nat Commun. 2018;9:1640 pubmed 出版商
Hruska M, Henderson N, Le Marchand S, Jafri H, Dalva M. Synaptic nanomodules underlie the organization and plasticity of spine synapses. Nat Neurosci. 2018;21:671-682 pubmed 出版商
O Neill A, Kyrousi C, Einsiedler M, Burtscher I, Drukker M, Markie D, et al. Mob2 Insufficiency Disrupts Neuronal Migration in the Developing Cortex. Front Cell Neurosci. 2018;12:57 pubmed 出版商
Huels D, Bruens L, Hodder M, Cammareri P, Campbell A, Ridgway R, et al. Wnt ligands influence tumour initiation by controlling the number of intestinal stem cells. Nat Commun. 2018;9:1132 pubmed 出版商
Je B, Xu F, Wu Q, Liu L, Meeley R, Gallagher J, et al. The CLAVATA receptor FASCIATED EAR2 responds to distinct CLE peptides by signaling through two downstream effectors. elife. 2018;7: pubmed 出版商
Lin M, Schöneberg J, Davies C, Ren X, Hurley J. The dynamic Atg13-free conformation of the Atg1 EAT domain is required for phagophore expansion. Mol Biol Cell. 2018;: pubmed 出版商
Yayon N, Dudai A, Vrieler N, Amsalem O, London M, Soreq H. Intensify3D: Normalizing signal intensity in large heterogenic image stacks. Sci Rep. 2018;8:4311 pubmed 出版商
Miotto B, Marchal C, Adelmant G, Guinot N, Xie P, Marto J, et al. Stabilization of the methyl-CpG binding protein ZBTB38 by the deubiquitinase USP9X limits the occurrence and toxicity of oxidative stress in human cells. Nucleic Acids Res. 2018;46:4392-4404 pubmed 出版商
Nichol H, Amilhon B, Manseau F, Badrinarayanan S, Williams S. Electrophysiological and Morphological Characterization of Chrna2 Cells in the Subiculum and CA1 of the Hippocampus: An Optogenetic Investigation. Front Cell Neurosci. 2018;12:32 pubmed 出版商
Crowther A, Lim S, Asrican B, Albright B, Wooten J, Yeh C, et al. An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus. Stem Cell Reports. 2018;10:1146-1159 pubmed 出版商
Ing Esteves S, Kostadinov D, Marocha J, Sing A, Joseph K, Laboulaye M, et al. Combinatorial Effects of Alpha- and Gamma-Protocadherins on Neuronal Survival and Dendritic Self-Avoidance. J Neurosci. 2018;38:2713-2729 pubmed 出版商
Li Y, Tian X, Zhao H, He L, Zhang S, Huang X, et al. Genetic targeting of Purkinje fibres by Sema3a-CreERT2. Sci Rep. 2018;8:2382 pubmed 出版商
Asokan P, Mitra R, Periasamy R, Han Z, BORRAS T. A Naturally Fluorescent Mgp Transgenic Mouse for Angiogenesis and Glaucoma Longitudinal Studies. Invest Ophthalmol Vis Sci. 2018;59:746-756 pubmed 出版商
Dillard C, Narbonne Reveau K, Foppolo S, Lanet E, Maurange C. Two distinct mechanisms silence chinmo in Drosophila neuroblasts and neuroepithelial cells to limit their self-renewal. Development. 2018;145: pubmed 出版商
Mitew S, Gobius I, Fenlon L, McDougall S, Hawkes D, Xing Y, et al. Pharmacogenetic stimulation of neuronal activity increases myelination in an axon-specific manner. Nat Commun. 2018;9:306 pubmed 出版商
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Ojala D, Sun S, Santiago Ortiz J, Shapiro M, Romero P, Schaffer D. In Vivo Selection of a Computationally Designed SCHEMA AAV Library Yields a Novel Variant for Infection of Adult Neural Stem Cells in the SVZ. Mol Ther. 2018;26:304-319 pubmed 出版商
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Hamada N, Iwamoto I, Tabata H, Nagata K. MUNC18-1 gene abnormalities are involved in neurodevelopmental disorders through defective cortical architecture during brain development. Acta Neuropathol Commun. 2017;5:92 pubmed 出版商
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Homsi Y, Lang T. The specificity of homomeric clustering of CD81 is mediated by its ?-loop. FEBS Open Bio. 2017;7:274-283 pubmed 出版商
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图像 1 :
Rockland Immunochemicals 600-401-379-RTU 图像 1
Western Blot of Ready-to-Use Rabbit Anti-RFP Antibody. Lane 1: Opal Prestain Molecular Weight (p/n MB-210-0500). Lane 2: HeLa (p/n W09-000-364). Lane 3: HEK293 (p/n W09-000-365). Lane 4: Cho/K1 (p/n W07-000-359). Lane 5: MDA-MB-231 (p/n W09-001-GK6). Lane 6: A431 (p/n W09-000-361). Lane 7: Jurkat (p/n W09-001-370). Lane 8: NIH/3T3 (p/n W10-000-358). Lane 9: E. coli HCP (p/n 000-001-J08). Lane 10: FLAG (p/n W00-001-383). Lane 11: RFP (p/n 000-001-379). Lane 12: GFP (p/n 000-001-215). Lane 13: GST (p/n 000-001-200). Lane 14: MBP (p/n 000-001-385). Lane 15: Opal Prestain. Primary Antibody: RTU-RFP at 1µL/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n 611-103-122) at 1:70,000 for 30min at RT. Expect 27kDa seen in lane 11. Unspecific band in lane 3 caused by cross reactivity with secondary antibody.
目录号 :
名称 :
Ready-To-Use Anti-RFP (RABBIT) Antibody Min X Hu Ms and Rt Serum Proteins - 600-401-379-RTU
显示名称 :
Ready-To-Use RFP Antibody Pre-adsorbed
应用注释 :
Ready-To-Use Anti-RFP is designed to detect RFP and its variants. Ready-To-Use Anti-RFP Rabbit Polyclonal Antibody has been optimized and validated in western blot using 1:1000 dilution. This Anti-RFP (RTU) Antibody is sufficient to run 10 western blots. Although not tested, this antibody is likely functional in ELISA, immunohistochemistry, immunofluorescence, and immunoprecipitation. Optimal titers for these applications should be determined by the researcher.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
浓度值 :
sufficient to run 10 immunoblot tests
共轭 :
规格 :
默认单元 :
ELISA稀释浓度 :
User Optimized
免疫组化稀释度 :
User Optimized
免疫荧光显微镜稀释浓度 :
User Optimized
免疫沉淀稀释度 :
User Optimized
免疫印迹稀释浓度 :
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
基因 :
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
包装类型 :
Wet Ice
物理状态 :
Liquid (sterile filtered)
防腐剂 :
纯度和特异性 :
RTU Anti-RFP was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma).  No reaction was observed against Human, Mouse or Rat serum proteins.
稳定剂 :
0.01% 牛血清白蛋白(BSA), 25%(v/v)甘油
储存 :
Store vial at 2-8° C prior to opening. May aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to use.
同义词 :
DsRed protein, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583, sea anemone Discosoma sp. Mushroom, RFP antibody, RFP-RTU, Ready To Use Antibody, RTU Antibody
背景 :
Fluorescent proteins such as green fluorescent protein (GFP) or Discosoma Red Fluorescent Protein (or DsRFP) are widely used in research practice. DsRed is isolated from sea anemone Discosoma sp. mushroom. GFP and DsRed share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, DsRed protein is similar to GFP protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, GFP protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Using site-directed mutagenesis, several DsRed protein variants have been created allowing the red fluorescent protein mature as a monomeric form. Among DsRFP monomeric variants are monomeric mutant mRFP1, mBanana, mCherry, mHoneydew, mPlum, mOrange, mStrawberry and mTangerine that offer a wide range of fluorescent colors. As Rockland RFP polyclonal antibodies are raised against whole RFP protein of wild type, the polyclonal antibodies are expected to recognize all RFP variant forms. This RFP antibody has been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein. All Rockland Immunochemical’s RFP antibodies are affinity-purified to assure both high affinity and specificity. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays.
表位标签类型 :
免疫原类型 :
低内毒素 :
样品尺寸 :
应用文本 :
酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫沉淀, 免疫印迹
类别 :
共轭名称 :
2022价格 :
最初图像名称 :
Western Blot of Ready-to-Use Rabbit Anti-RFP Antibody
更多信息或购买 :
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。