产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
蛋白
产品名称 :
Ready-To-Use RFP Antibody Pre-adsorbed
目录 :
600-401-379-RTU
规格 :
100
价格 :
214.00美元
更多信息或购买 :
文章摘录数: 169
参考文献
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图像
图像 1 :
Rockland Immunochemicals 600-401-379-RTU 图像 1
Western Blot of Ready-to-Use Rabbit Anti-RFP Antibody. Lane 1: Opal Prestain Molecular Weight (p/n MB-210-0500). Lane 2: HeLa (p/n W09-000-364). Lane 3: HEK293 (p/n W09-000-365). Lane 4: Cho/K1 (p/n W07-000-359). Lane 5: MDA-MB-231 (p/n W09-001-GK6). Lane 6: A431 (p/n W09-000-361). Lane 7: Jurkat (p/n W09-001-370). Lane 8: NIH/3T3 (p/n W10-000-358). Lane 9: E. coli HCP (p/n 000-001-J08). Lane 10: FLAG (p/n W00-001-383). Lane 11: RFP (p/n 000-001-379). Lane 12: GFP (p/n 000-001-215). Lane 13: GST (p/n 000-001-200). Lane 14: MBP (p/n 000-001-385). Lane 15: Opal Prestain. Primary Antibody: RTU-RFP at 1µL/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n 611-103-122) at 1:70,000 for 30min at RT. Expect 27kDa seen in lane 11. Unspecific band in lane 3 caused by cross reactivity with secondary antibody.
产品信息
目录号 :
600-401-379-RTU
名称 :
Ready-To-Use Anti-RFP (RABBIT) Antibody Min X Hu Ms and Rt Serum Proteins - 600-401-379-RTU
显示名称 :
Ready-To-Use RFP Antibody Pre-adsorbed
应用注释 :
Ready-To-Use Anti-RFP is designed to detect RFP and its variants. Ready-To-Use Anti-RFP Rabbit Polyclonal Antibody has been optimized and validated in western blot using 1:1000 dilution. This Anti-RFP (RTU) Antibody is sufficient to run 10 western blots. Although not tested, this antibody is likely functional in ELISA, immunohistochemistry, immunofluorescence, and immunoprecipitation. Optimal titers for these applications should be determined by the researcher.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
sufficient to run 10 immunoblot tests
共轭 :
(None)
规格 :
100
默认单元 :
微升
ELISA稀释浓度 :
User Optimized
免疫组化稀释度 :
User Optimized
免疫荧光显微镜稀释浓度 :
User Optimized
免疫沉淀稀释度 :
User Optimized
免疫印迹稀释浓度 :
1:1,000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG
基因 :
DsRed
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
包装类型 :
Wet Ice
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
RTU Anti-RFP was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma).  No reaction was observed against Human, Mouse or Rat serum proteins.
稳定剂 :
0.01% 牛血清白蛋白(BSA), 25%(v/v)甘油
储存 :
Store vial at 2-8° C prior to opening. May aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to use.
同义词 :
DsRed protein, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583, sea anemone Discosoma sp. Mushroom, RFP antibody, RFP-RTU, Ready To Use Antibody, RTU Antibody
背景 :
Fluorescent proteins such as green fluorescent protein (GFP) or Discosoma Red Fluorescent Protein (or DsRFP) are widely used in research practice. DsRed is isolated from sea anemone Discosoma sp. mushroom. GFP and DsRed share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, DsRed protein is similar to GFP protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, GFP protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Using site-directed mutagenesis, several DsRed protein variants have been created allowing the red fluorescent protein mature as a monomeric form. Among DsRFP monomeric variants are monomeric mutant mRFP1, mBanana, mCherry, mHoneydew, mPlum, mOrange, mStrawberry and mTangerine that offer a wide range of fluorescent colors. As Rockland RFP polyclonal antibodies are raised against whole RFP protein of wild type, the polyclonal antibodies are expected to recognize all RFP variant forms. This RFP antibody has been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein. All Rockland Immunochemical’s RFP antibodies are affinity-purified to assure both high affinity and specificity. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays.
表位标签类型 :
RFP
免疫原类型 :
重组蛋白
低内毒素 :
No
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫沉淀, 免疫印迹
类别 :
绿色荧光蛋白和表位
共轭名称 :
未共轭
2022价格 :
214.00美元
最初图像名称 :
Western Blot of Ready-to-Use Rabbit Anti-RFP Antibody
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。