产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
RFP Antibody Pre-adsorbed
目录 :
600-401-379
规格 :
100
价格 :
https://www.rockland.com/search/?searchString=600-401-379
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 837
出版应用/物种/样本/稀释参考文献
  • 免疫组化; 小鼠; 1:200; 图 4i
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  • 免疫组化-石蜡切片; 小鼠; 1:250; 图 5b
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  • 免疫组化-石蜡切片; 小鼠; 1:500; 图 s1e
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  • 免疫组化; 小鼠; 1:1000; 图 1
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产品信息
目录号 :
600-401-379
名称 :
Anti-RFP (RABBIT) Antibody Min X Hu Ms and Rt Serum Proteins - 600-401-379
显示名称 :
RFP Antibody Pre-adsorbed
应用注释 :
Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody has been tested by ELISA, Western blot, IF, and IHC, and is suitable for use in EM, FC, FISH, IP, and multiplex assays based on published references. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Optimal titers for applications should be determined by the researcher.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
1.05毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100
默认单元 :
微克
ELISA稀释浓度 :
1:28, 700 - 1:48, 700
流式细胞仪稀释浓度 :
1:200 - 1:2, 000
免疫组化稀释度 :
1:200 - 1:2, 000
免疫荧光显微镜稀释浓度 :
1:200 - 1:2, 000
免疫沉淀稀释度 :
User Optimized
免疫印迹稀释浓度 :
1:1, 000 - 1:5, 000
有效期 :
Expiration date is one (1) year from date of receipt.
格式 :
IgG
基因名称 :
DsRed
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma).  No reaction was observed against Human, Mouse or Rat serum proteins.
物种活性 :
RFP; mScarlet; rRFP; tdTomato
检测过的应用 :
酶联免疫吸附测定;IF;免疫组化;免疫印迹
Suggested Applications :
EM;FC;鱼类;免疫沉淀;其他
储存 :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583.
背景 :
Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from the mushroom anemone Discosoma sp. or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion proteins with RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Rockland offers many controls, monoclonal, and polyclonal antibodies for RFP.
免疫原类型 :
重组蛋白
低内毒素 :
No
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, 流式细胞仪, 免疫组化, IF显微镜, 免疫沉淀, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2024价格 :
$299.00
UniProt数据库 :
Q9U6Y8
最初图像名称 :
免疫荧光-抗RFP抗体
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。