产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
Antibody for the detection of FLAG™ conjugated proteins
目录 :
600-401-383
规格 :
250
价格 :
https://www.rockland.com/search/?searchString=600-401-383
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀
更多信息或购买 :
Rockland Immunochemicals 产品网页
文章摘录数: 26
出版应用/物种/样本/稀释参考文献
  • 免疫组化; 小鼠; 1:5000; 图 3f
De Giorgi M, Li A, Hurley A, Barzi M, Doerfler A, Cherayil N, et al. Targeting the Apoa1 locus for liver-directed gene therapy. Mol Ther Methods Clin Dev. 2021;21:656-669 pubmed 出版商
H xe4 usl A, Bajaj T, Brix L, P xf6 hlmann M, Häfner K, De Angelis M, et al. Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolism. Sci Adv. 2022;8:eabi4797 pubmed 出版商
Fusi L, Paudel R, Meder K, Schlosser A, Schrama D, Goebeler M, et al. Interaction of transcription factor FoxO3 with histone acetyltransferase complex subunit TRRAP modulates gene expression and apoptosis. J Biol Chem. 2022;298:101714 pubmed 出版商
Haines A, Wesolowski J, Ryan N, Monteiro Br xe1 s T, Paumet F. Cross Talk between ARF1 and RhoA Coordinates the Formation of Cytoskeletal Scaffolds during Chlamydia Infection. MBio. 2021;12:e0239721 pubmed 出版商
Herrera Cruz M, Yap M, Tahbaz N, Phillips K, Thomas L, Thomas G, et al. Rab32 uses its effector reticulon 3L to trigger autophagic degradation of mitochondria-associated membrane (MAM) proteins. Biol Direct. 2021;16:22 pubmed 出版商
Martinelli S, Anderzhanova E, Bajaj T, Wiechmann S, Dethloff F, Weckmann K, et al. Stress-primed secretory autophagy promotes extracellular BDNF maturation by enhancing MMP9 secretion. Nat Commun. 2021;12:4643 pubmed 出版商
Nabar N, Heijjer C, Shi C, Hwang I, Ganesan S, Karlsson M, et al. LRRK2 is required for CD38-mediated NAADP-Ca2+ signaling and the downstream activation of TFEB (transcription factor EB) in immune cells. Autophagy. 2021;:1-19 pubmed 出版商
Li Z, Gu S, Quan Y, Varadaraj K, Jiang J. Development of a potent embryonic chick lens model for studying congenital cataracts in vivo. Commun Biol. 2021;4:325 pubmed 出版商
Carr H, Chang J, Frost J. The PDZ Domain Protein SYNJ2BP Regulates GRK-Dependent Sst2A Phosphorylation and Downstream MAPK Signaling. Endocrinology. 2021;162: pubmed 出版商
Chang C, Xu X, Li M, Xin D, Ding L, Wang Y, et al. Pathogenic mutations reveal a role of RECQ4 in mitochondrial RNA:DNA hybrid formation and resolution. Sci Rep. 2020;10:17033 pubmed 出版商
Lindner F, Milne Davies B, Langenfeld K, Stiewe T, Diepold A. LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution. Nat Commun. 2020;11:2381 pubmed 出版商
Sadek J, Wuo M, Rooklin D, Hauenstein A, Hong S, Gautam A, et al. Modulation of virus-induced NF-κB signaling by NEMO coiled coil mimics. Nat Commun. 2020;11:1786 pubmed 出版商
Skotnicka D, Steinchen W, Szadkowski D, Cadby I, Lovering A, Bange G, et al. CdbA is a DNA-binding protein and c-di-GMP receptor important for nucleoid organization and segregation in Myxococcus xanthus. Nat Commun. 2020;11:1791 pubmed 出版商
Zannas A, Jia M, Häfner K, Baumert J, Wiechmann T, Pape J, et al. Epigenetic upregulation of FKBP5 by aging and stress contributes to NF-κB-driven inflammation and cardiovascular risk. Proc Natl Acad Sci U S A. 2019;: pubmed 出版商
Olsen C, Memarzadeh K, Ulu A, Carr H, Bean A, Frost J. Regulation of Somatostatin Receptor 2 Trafficking by C-Tail Motifs and the Retromer. Endocrinology. 2019;160:1031-1043 pubmed 出版商
Terra L, Wailemann R, Dos Santos A, Gomes V, Silva R, Laporte A, et al. Heat shock protein B1 is a key mediator of prolactin-induced beta-cell cytoprotection against oxidative stress. Free Radic Biol Med. 2019;134:394-405 pubmed 出版商
Yue Y, Nabar N, Shi C, Kamenyeva O, Xiao X, Hwang I, et al. SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death. Cell Death Dis. 2018;9:904 pubmed 出版商
Shimo T, Tachibana K, Obika S. Construction of a tri-chromatic reporter cell line for the rapid and simple screening of splice-switching oligonucleotides targeting DMD exon 51 using high content screening. PLoS ONE. 2018;13:e0197373 pubmed 出版商
Geng S, Miyagi A, Umen J. Evolutionary divergence of the sex-determining gene MID uncoupled from the transition to anisogamy in volvocine algae. Development. 2018;145: pubmed 出版商
Lucarelli P, Schilling M, Kreutz C, Vlasov A, Boehm M, Iwamoto N, et al. Resolving the Combinatorial Complexity of Smad Protein Complex Formation and Its Link to Gene Expression. Cell Syst. 2018;6:75-89.e11 pubmed 出版商
Hu Z, Shi W, Riquelme M, Shi Q, Biswas S, Lo W, et al. Connexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation. Sci Rep. 2017;7:5298 pubmed 出版商
Smith T, Li J, Dores M, Trejo J. Protease-activated receptor-4 and purinergic receptor P2Y12 dimerize, co-internalize, and activate Akt signaling via endosomal recruitment of β-arrestin. J Biol Chem. 2017;292:13867-13878 pubmed 出版商
Smith T, Coronel L, Li J, Dores M, Nieman M, Trejo J. Protease-activated Receptor-4 Signaling and Trafficking Is Regulated by the Clathrin Adaptor Protein Complex-2 Independent of ?-Arrestins. J Biol Chem. 2016;291:18453-64 pubmed 出版商
Wang F, Alain T, Szretter K, Stephenson K, Pol J, Atherton M, et al. S6K-STING interaction regulates cytosolic DNA-mediated activation of the transcription factor IRF3. Nat Immunol. 2016;17:514-522 pubmed 出版商
Grimsey N, Coronel L, Cordova I, Trejo J. Recycling and Endosomal Sorting of Protease-activated Receptor-1 Is Distinctly Regulated by Rab11A and Rab11B Proteins. J Biol Chem. 2016;291:2223-36 pubmed 出版商
Gassen N, Hartmann J, Zannas A, Kretzschmar A, Zschocke J, Maccarrone G, et al. FKBP51 inhibits GSK3β and augments the effects of distinct psychotropic medications. Mol Psychiatry. 2016;21:277-89 pubmed 出版商
产品信息
目录号 :
600-401-383
名称 :
Antibody for the detection of FLAG™ conjugated proteins (RABBIT) - 600-401-383
显示名称 :
Antibody for the detection of FLAG™ conjugated proteins
应用注释 :
This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins.  As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope.  The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins.  This antibody has been tested by ELISA and western blotting against both the immunizing peptide and FLAGä containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques.  The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7.  Now the most commonly used hydrophilic octapeptide is DYKDDDDK.  Rockland Immunochemical's polyclonal antibody to detect FLAG™ conjugated proteins binds FLAG™ containing fusion proteins with greater affinity than the widely used monoclonal M1, M2 and M5 clones, and shows greater sensitivity in most assays.  Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
1.13毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
250
默认单元 :
微克
ELISA稀释浓度 :
1:20, 000 - 1:40, 000
免疫组化稀释度 :
User Optimized
免疫印迹稀释浓度 :
1:2, 000 - 1:10, 000
有效期 :
Expiration date is one (1) year from date of receipt.
格式 :
IgG
宿主动物 :
兔
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.  This antibody reacts with FLAG™ conjugated proteins.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
This affinity purified antibody is directed against the FLAG™ motif and is useful in determining its presence in various assays. This polyclonal anti-FLAG™ tag antibody detects over-expressed proteins containing the FLAG™ epitope tag.  In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
物种活性 :
FLAG标签标签(C末端)
检测过的应用 :
酶联免疫吸附测定;免疫印迹
Suggested Applications :
IF; IHC; IP; Multiplex
储存 :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
rabbit antibody for the detection of FLAG™ conjugated proteins, rabbit anti DYKDDDDK
背景 :
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein.  Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
免疫原类型 :
共轭标签肽
低内毒素 :
No
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, 免疫组化, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2024价格 :
$233.00
最初图像名称 :
FLAG™共轭标签蛋白-免疫印迹
更多信息或购买 :
Rockland Immunochemicals 产品网页
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部，为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立，利用In vitro及In vivo方法制备并纯化单抗及多抗，这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究：Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。