产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
血球凝集素表位标签抗体
目录 :
600-401-384
规格 :
100
价格 :
https://www.rockland.com/search/?searchString=600-401-384
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 染色质免疫沉淀
更多信息或购买 :
Rockland Immunochemicals 产品网页
文章摘录数: 45
参考文献
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May J, Johnson P, Ilyas M, Gao F, Simon A. The Multifunctional Long-Distance Movement Protein of Pea Enation Mosaic Virus 2 Protects Viral and Host Transcripts from Nonsense-Mediated Decay. MBio. 2020;11: pubmed 出版商
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Rohde C, Becker S, Krähling V. Marburg virus regulates the IRE1/XBP1-dependent unfolded protein response to ensure efficient viral replication. Emerg Microbes Infect. 2019;8:1300-1313 pubmed 出版商
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产品信息
目录号 :
600-401-384
名称 :
抗血球凝集素EPITOPE标签(兔)抗体-600-401
显示名称 :
血球凝集素表位标签抗体
应用注释 :
Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins.  As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope.  The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors.  This antibody has been tested by ELISA, immunohistochemistry, and western blotting against both the immunizing peptide and HA containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques.  Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
1.05毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100
默认单元 :
微克
ELISA稀释浓度 :
1:10, 000 - 1:100, 000
染色质免疫共沉淀稀释 :
User Optimized
免疫组化稀释度 :
1:500 - 1:2, 000
免疫沉淀稀释度 :
User Optimized
免疫印迹稀释浓度 :
1:2, 000 - 1:10, 000
有效期 :
Expiration date is one (1) year from date of receipt.
格式 :
IgG
宿主动物 :
兔
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-HA antibody was purified from whole rabbit serum prepared by repeated immunizations with the epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag.  To date, this antibody has reacted with all HA-tagged proteins tested.  In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
物种活性 :
血球凝集素标签
检测过的应用 :
酶联免疫吸附测定;免疫组化;免疫印迹
Suggested Applications :
IF; IP; Multiplex
储存 :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
兔抗血球凝集素表位标签抗体, 兔抗血凝素抗体, 兔抗血球凝集素标签抗体, 抗表位
背景 :
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein.   Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.
免疫原类型 :
共轭标签肽
低内毒素 :
No
样品尺寸 :
No
应用文本 :
ELISA,Chip,Immunohistochemistry,Immunoprecipitation,Western Blot,
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2024价格 :
$299.00
最初图像名称 :
抗血球凝集素表位标签抗体-免疫组化
更多信息或购买 :
Rockland Immunochemicals 产品网页
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部，为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立，利用In vitro及In vivo方法制备并纯化单抗及多抗，这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究：Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。