产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
beta肌动蛋白抗体
目录 :
600-401-886
规格 :
200微克
价格 :
308.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 酶联免疫吸附测定
更多信息或购买 :
文章摘录数: 13
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 1:500; 图 6
Campos Y, Qiu X, Gomero E, Wakefield R, Horner L, Brutkowski W, et al. Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier. Nat Commun. 2016;7:11876 pubmed 出版商
  • 免疫印迹; 人类; 图 1
CARR F, Tai P, Barnum M, Gillis N, Evans K, Taber T, et al. Thyroid Hormone Receptor-? (TR?) Mediates Runt-Related Transcription Factor 2 (Runx2) Expression in Thyroid Cancer Cells: A Novel Signaling Pathway in Thyroid Cancer. Endocrinology. 2016;157:3278-92 pubmed 出版商
Rosas Ballina M, Guan X, Schmidt A, Bumann D. Classical Activation of Macrophages Leads to Lipid Droplet Formation Without de novo Fatty Acid Synthesis. Front Immunol. 2020;11:131 pubmed 出版商
Olive J, Qin Y, Decristo M, Laszewski T, Greathouse F, McAllister S. Accounting for tumor heterogeneity when using CRISPR-Cas9 for cancer progression and drug sensitivity studies. PLoS ONE. 2018;13:e0198790 pubmed 出版商
Borroni A, Emanuelli A, Shah P, Ilic N, Apel Sarid L, Paolini B, et al. Smurf2 regulates stability and the autophagic-lysosomal turnover of lamin A and its disease-associated form progerin. Aging Cell. 2018;17: pubmed 出版商
Coleman M, Ha S, Haeryfar S, Barr S, Kim S. Cathepsin B plays a key role in optimal production of the influenza A virus. J Virol Antivir Res. 2018;2018:1-20 pubmed 出版商
Lodola F, Laforenza U, Cattaneo F, Ruffinatti F, Poletto V, Massa M, et al. VEGF-induced intracellular Ca2+ oscillations are down-regulated and do not stimulate angiogenesis in breast cancer-derived endothelial colony forming cells. Oncotarget. 2017;8:95223-95246 pubmed 出版商
Chen X, Wang W, Man H, Li P, Shan B. Increased B7-H4 expression during esophageal squamous cell carcinogenesis is associated with IL-6/STAT3 signaling pathway activation in mice. Oncol Lett. 2017;13:2207-2215 pubmed 出版商
Maragkoudaki M, Vaiopoulou A, Theodoropoulos G, Legaki E, Sechi L, Karamanolis G, et al. Specific detection of OCT4 isoforms in inflammatory bowel disease. Gut Pathog. 2015;7:25 pubmed 出版商
Rae D, Hocum J, Bii V, Deeg H, Trobridge G. A novel retroviral mutagenesis screen identifies prognostic genes in RUNX1 mediated myeloid leukemogenesis. Oncotarget. 2015;6:30664-74 pubmed 出版商
Koh D, Powell D, Blake S, Hoffman J, Hopkins M, Feng X. Enhanced cytotoxicity in triple-negative and estrogen receptor‑positive breast adenocarcinoma cells due to inhibition of the transient receptor potential melastatin-2 channel. Oncol Rep. 2015;34:1589-98 pubmed 出版商
Hopkins M, Feng X, Liu M, Parker L, Koh D. Inhibition of the transient receptor potential melastatin-2 channel causes increased DNA damage and decreased proliferation in breast adenocarcinoma cells. Int J Oncol. 2015;46:2267-76 pubmed 出版商
Dragoni S, Turin I, Laforenza U, Potenza D, Bottino C, Glasnov T, et al. Store-operated Ca2+ entry does not control proliferation in primary cultures of human metastatic renal cellular carcinoma. Biomed Res Int. 2014;2014:739494 pubmed 出版商
产品信息
目录号 :
600-401-886
名称 :
抗beta肌动蛋白(兔)抗体-600-401
显示名称 :
beta肌动蛋白抗体
应用注释 :
Anti-beta Actin Antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
1.0毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
200微克
默认单元 :
微克
ELISA稀释浓度 :
1:10, 000 - 1:40, 000
免疫荧光显微镜稀释浓度 :
1:500 - 1:2, 000
免疫印迹稀释浓度 :
1:1, 000 - 1:4, 000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG
基因名称 :
ACTB
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
Anti-beta-Actin Loading Control Antibody reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein.  A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
物种活性 :
Mouse, Human, Leopard frog
储存 :
Store beta-Actin Loading Control Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody
靶物种 :
人类
背景 :
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
免疫原类型 :
多肽
低内毒素 :
No
样品尺寸 :
No
靶活性结构域 :
C末端
应用文本 :
酶联免疫吸附测定, IF显微镜, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2020价格 :
308.00美元
UniProt数据库 :
P60709
NCBI :
4501885
最初图像名称 :
抗beta肌动蛋白抗体-免疫组化
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。