产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
组蛋白H2AvD phosphoS137抗体
目录 :
600-401-914
规格 :
100
价格 :
https://www.rockland.com/search/?searchString=600-401-914
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
抗原修饰 :
磷酸化
反应物种 :
fruit fly
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化
更多信息或购买 :
文章摘录数: 44
出版应用/物种/样本/稀释参考文献
  • 免疫组化; fruit fly ; 1:500; 图 s2
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Liu J, Tao X, Zhu Y, Li C, Ruan K, Diaz Perez Z, et al. NMNAT promotes glioma growth through regulating post-translational modifications of P53 to inhibit apoptosis. elife. 2021;10: pubmed 出版商
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Lin K, Wang W, Lin C, Rastegari E, Su Y, Chang Y, et al. Piwi reduction in the aged niche eliminates germline stem cells via Toll-GSK3 signaling. Nat Commun. 2020;11:3147 pubmed 出版商
Na H, Akan I, Abramowitz L, Hanover J. Nutrient-Driven O-GlcNAcylation Controls DNA Damage Repair Signaling and Stem/Progenitor Cell Homeostasis. Cell Rep. 2020;31:107632 pubmed 出版商
Rastegari E, Kajal K, Tan B, Huang F, Chen R, Hsieh T, et al. WD40 protein Wuho controls germline homeostasis via TRIM-NHL tumor suppressor Mei-p26 in Drosophila. Development. 2020;147: pubmed 出版商
Mascolo E, Amoroso N, Saggio I, Merigliano C, Vernì F. Pyridoxine/pyridoxamine 5'-phosphate oxidase (Sgll/PNPO) is important for DNA integrity and glucose homeostasis maintenance in Drosophila. J Cell Physiol. 2020;235:504-512 pubmed 出版商
Nano M, Gemble S, Simon A, Pennetier C, Fraisier V, Marthiens V, et al. Cell-Cycle Asynchrony Generates DNA Damage at Mitotic Entry in Polyploid Cells. Curr Biol. 2019;29:3937-3945.e7 pubmed 出版商
Perlmutter J, Bordenstein S, Unckless R, LePage D, Metcalf J, Hill T, et al. The phage gene wmk is a candidate for male killing by a bacterial endosymbiont. PLoS Pathog. 2019;15:e1007936 pubmed 出版商
Chang Y, Dubnau J. The Gypsy Endogenous Retrovirus Drives Non-Cell-Autonomous Propagation in a Drosophila TDP-43 Model of Neurodegeneration. Curr Biol. 2019;29:3135-3152.e4 pubmed 出版商
Dialynas G, Delabaere L, Chiolo I. Arp2/3 and Unc45 maintain heterochromatin stability in Drosophila polytene chromosomes. Exp Biol Med (Maywood). 2019;244:1362-1371 pubmed 出版商
Grendler J, Lowgren S, Mills M, Losick V. Wound-induced polyploidization is driven by Myc and supports tissue repair in the presence of DNA damage. Development. 2019;146: pubmed 出版商
Murcia L, Clemente Ruiz M, Pierre Elies P, Royou A, Milan M. Selective Killing of RAS-Malignant Tissues by Exploiting Oncogene-Induced DNA Damage. Cell Rep. 2019;28:119-131.e4 pubmed 出版商
Tsakiri E, Gumeni S, Vougas K, Pendin D, Papassideri I, Daga A, et al. Proteasome dysfunction induces excessive proteome instability and loss of mitostasis that can be mitigated by enhancing mitochondrial fusion or autophagy. Autophagy. 2019;15:1757-1773 pubmed 出版商
Okumura K, Nishihara S, Inoue Y. Genetic identification and characterization of three genes that prevent accumulation of oxidative DNA damage in Drosophila adult tissues. DNA Repair (Amst). 2019;78:7-19 pubmed 出版商
Cosolo A, Jaiswal J, Csordás G, Grass I, Uhlirova M, Classen A. JNK-dependent cell cycle stalling in G2 promotes survival and senescence-like phenotypes in tissue stress. elife. 2019;8: pubmed 出版商
Merigliano C, Mascolo E, Cesta A, Saggio I, Vernì F. A new role for Drosophila Aurora-A in maintaining chromosome integrity. Chromosoma. 2019;128:41-52 pubmed 出版商
McCarthy A, Deiulio A, Martin E, Upadhyay M, Rangan P. Tip60 complex promotes expression of a differentiation factor to regulate germline differentiation in female Drosophila. Mol Biol Cell. 2018;29:2933-2945 pubmed 出版商
Clémot M, Molla Herman A, Mathieu J, Huynh J, Dostatni N. The replicative histone chaperone CAF1 is essential for the maintenance of identity and genome integrity in adult stem cells. Development. 2018;145: pubmed 出版商
Merigliano C, Mascolo E, La Torre M, Saggio I, Vernì F. Protective role of vitamin B6 (PLP) against DNA damage in Drosophila models of type 2 diabetes. Sci Rep. 2018;8:11432 pubmed 出版商
Mlih M, Khericha M, Birdwell C, West A, Karpac J. A virus-acquired host cytokine controls systemic aging by antagonizing apoptosis. PLoS Biol. 2018;16:e2005796 pubmed 出版商
Caridi C, D Agostino C, Ryu T, Zapotoczny G, Delabaere L, Li X, et al. Nuclear F-actin and myosins drive relocalization of heterochromatic breaks. Nature. 2018;559:54-60 pubmed 出版商
Harumoto T, Lemaitre B. Male-killing toxin in a bacterial symbiont of Drosophila. Nature. 2018;557:252-255 pubmed 出版商
Feng L, Shi Z, Xie J, Ma B, Chen X. Enhancer of polycomb maintains germline activity and genome integrity in Drosophila testis. Cell Death Differ. 2018;25:1486-1502 pubmed 出版商
Guarner A, Morris R, Korenjak M, Boukhali M, Zappia M, Van Rechem C, et al. E2F/DP Prevents Cell-Cycle Progression in Endocycling Fat Body Cells by Suppressing dATM Expression. Dev Cell. 2017;43:689-703.e5 pubmed 出版商
Bayona Feliu A, Casas Lamesa A, Reina O, Bernues J, Azorín F. Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin. Nat Commun. 2017;8:283 pubmed 出版商
Colmenares S, Swenson J, Langley S, Kennedy C, Costes S, Karpen G. Drosophila Histone Demethylase KDM4A Has Enzymatic and Non-enzymatic Roles in Controlling Heterochromatin Integrity. Dev Cell. 2017;42:156-169.e5 pubmed 出版商
Koehler C, Perkins G, Ellisman M, Jones D. Pink1 and Parkin regulate Drosophila intestinal stem cell proliferation during stress and aging. J Cell Biol. 2017;216:2315-2327 pubmed 出版商
Ma X, Zhu X, Han Y, Story B, Do T, Song X, et al. Aubergine Controls Germline Stem Cell Self-Renewal and Progeny Differentiation via Distinct Mechanisms. Dev Cell. 2017;41:157-169.e5 pubmed 出版商
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Hong S, Choi K. Antagonistic roles of Drosophila Tctp and Brahma in chromatin remodelling and stabilizing repeated sequences. Nat Commun. 2016;7:12988 pubmed 出版商
Swenson J, Colmenares S, Strom A, Costes S, Karpen G. The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic. elife. 2016;5: pubmed 出版商
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Chen H, Zheng X, Xiao D, Zheng Y. Age-associated de-repression of retrotransposons in the Drosophila fat body, its potential cause and consequence. Aging Cell. 2016;15:542-52 pubmed 出版商
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产品信息
目录号 :
600-401-914
名称 :
抗组蛋白H2AvD pS137(兔)抗体-600-401
显示名称 :
组蛋白H2AvD phosphoS137抗体
应用注释 :
Histone H2AvD pS137 Antibody is tested in ELISA, Immunohistochemistry, and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract.  Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pS137 of H2AvD protein.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
1.1毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100
默认单元 :
微克
ELISA稀释浓度 :
1:29, 000 - 1:30, 000
免疫组化稀释度 :
2微克/毫升
免疫印迹稀释浓度 :
1:400 - 1:1, 600
有效期 :
Expiration date is one (1) year from date of receipt.
格式 :
IgG
基因名称 :
His2Av
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Histone H2AvD pS137 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 125-141 of Drosophila melanogaster (fruit fly) H2AvD protein.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
Affinity purified Anti-Histone H2AvD pS137 Antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the pS137 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.  Reactivity occurs against Drosophila H2AvD pS137 protein and the antibody is specific for the phosphorylated form of the protein.   Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA.  A BLAST analysis was used to suggest little to no cross reactivity with H2AvD proteins from other sources based on a comparison using the immunizing sequence.  Reactivity against homologues from other sources is not known.
物种活性 :
D. melanogaster
检测过的应用 :
酶联免疫吸附测定;免疫组化;免疫印迹
Suggested Applications :
ChIP; EM; IF; Multiplex
储存 :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
兔抗H2AvD pS137抗体, 兔抗组蛋白H2A.v pS137抗体, H2AvD蛋白抗体, H2A.F/Z, H2A.Z, H2AvD, His2AvD, His2Av
靶物种 :
黑腹果蝇
背景 :
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known.  H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD  is expressed both maternally and zygotically and is found in embryos through to adults (female only).  The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur.  In mouse, H2AX ''knock out'' mice have an increased incidence of cancer.
免疫原类型 :
共轭标签肽
低内毒素 :
No
翻译后的修饰作用 :
磷酸化
样品尺寸 :
No
靶活性结构域 :
C末端
应用文本 :
酶联免疫吸附测定, 免疫组化, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2024价格 :
$475.00
UniProt数据库 :
P08985
NCBI :
17738227
最初图像名称 :
抗组蛋白H2AvD pS137抗体-免疫组化
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。