产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
SMAD3磷酸化S423/磷酸化S425抗体
目录 :
600-401-919
规格 :
100微克
价格 :
369.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 11
出版应用/物种/样本/稀释参考文献
  • 免疫组化-石蜡切片; 小鼠; 1:1000; 图 6e
Su Q, Zhang B, Zhang L, Dang T, Rowley D, Ittmann M, et al. Jagged1 upregulation in prostate epithelial cells promotes formation of reactive stroma in the Pten null mouse model for prostate cancer. Oncogene. 2017;36:618-627 pubmed 出版商
Chung S, Overstreet J, Li Y, Wang Y, Niu A, Wang S, et al. TGF-β promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration. JCI Insight. 2018;3: pubmed 出版商
Tang Y, Tang L, Xu X, Li C, Deng C, Zhang Y. Generation of Smurf2 Conditional Knockout Mice. Int J Biol Sci. 2018;14:542-548 pubmed 出版商
Li Y, Chung S, Li Z, Overstreet J, Gagnon L, Grouix B, et al. Fatty acid receptor modulator PBI-4050 inhibits kidney fibrosis and improves glycemic control. JCI Insight. 2018;3: pubmed 出版商
Feng M, Tang P, Huang X, Sun S, You Y, Xiao J, et al. TGF-β Mediates Renal Fibrosis via the Smad3-Erbb4-IR Long Noncoding RNA Axis. Mol Ther. 2018;26:148-161 pubmed 出版商
Li J, Shi S, Srivastava S, Kitada M, Nagai T, Nitta K, et al. FGFR1 is critical for the anti-endothelial mesenchymal transition effect of N-acetyl-seryl-aspartyl-lysyl-proline via induction of the MAP4K4 pathway. Cell Death Dis. 2017;8:e2965 pubmed 出版商
Tang L, Heller M, Meng Z, Yu L, Tang Y, Zhou M, et al. Transforming Growth Factor-β (TGF-β) Directly Activates the JAK1-STAT3 Axis to Induce Hepatic Fibrosis in Coordination with the SMAD Pathway. J Biol Chem. 2017;292:4302-4312 pubmed 出版商
Subathra M, Korrapati M, Howell L, Arthur J, Shayman J, Schnellmann R, et al. Kidney glycosphingolipids are elevated early in diabetic nephropathy and mediate hypertrophy of mesangial cells. Am J Physiol Renal Physiol. 2015;309:F204-15 pubmed 出版商
Luo Y, Xu W, Chen H, Warburton D, Dong R, Qian B, et al. A novel profibrotic mechanism mediated by TGFβ-stimulated collagen prolyl hydroxylase expression in fibrotic lung mesenchymal cells. J Pathol. 2015;236:384-94 pubmed 出版商
Herhaus L, Al Salihi M, MacArtney T, Weidlich S, Sapkota G. OTUB1 enhances TGF? signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3. Nat Commun. 2013;4:2519 pubmed 出版商
Yamashita M, Fatyol K, Jin C, Wang X, Liu Z, Zhang Y. TRAF6 mediates Smad-independent activation of JNK and p38 by TGF-beta. Mol Cell. 2008;31:918-24 pubmed 出版商
产品信息
目录号 :
600-401-919
名称 :
抗SMAD3 pS423 pS425(兔)抗体-600-401
显示名称 :
SMAD3磷酸化S423/磷酸化S425抗体
应用注释 :
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.  Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
1.1毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100微克
默认单元 :
微克
ELISA稀释浓度 :
1:15, 000 - 1:30, 000
流式细胞仪稀释浓度 :
User Optimized
免疫组化稀释度 :
1:500
免疫印迹稀释浓度 :
1:2, 000 - 1:20, 000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG
基因名称 :
SMAD3
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-SMAD3 pS423pS425 antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human SMAD3 protein.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.  Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein.   Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot.  Expect reactivity against phosphorylated Smad1 and Smad5.  Negligible reactivity is seen against other phosphorylated Smad family members.  A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen.  Reactivity against homologues from other sources is not known.
物种活性 :
人类
储存 :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
rabbit anti-SMAD3 pS423pS425 antibody, SMAD-3, SMAD 3, mothers against decapentaplegic homolog 3 antibody, MAD homolog 3, Mothers against DPP homolog 3, SMAD family member 3, MADH3, MADH 3, JV15-2
靶物种 :
人类
背景 :
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase.   These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily.  Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway.  Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes.  Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
免疫原类型 :
多肽
低内毒素 :
No
翻译后的修饰作用 :
磷酸化
样品尺寸 :
No
靶活性结构域 :
内部
应用文本 :
酶联免疫吸附测定, 流式细胞仪, 免疫组化, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2020价格 :
369.00美元
UniProt数据库 :
P84022
NCBI :
5174513
最初图像名称 :
抗Smad3 pS423 pS425抗体-免疫组化
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。