抗体

p44地图激酶抗体

600-401-943

100微克

多克隆

domestic rabbit

未共轭

人类, 小鼠

免疫印迹, 酶联免疫吸附测定, 免疫沉淀

抗p44地图激酶(ERK1)(兔)抗体-600-401

p44地图激酶抗体

This affinity purified antibody has been tested for use in ELISA, immunoprecipitation and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 44 kDa in size corresponding to p44 MAP Kinase (ERK1) by western blotting in the appropriate cell lysate or extract.

0.1 M三羟甲基氨基甲烷氯化物, 0.5 M氯化钠, pH值8.0

多克隆

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:2,000 to 1:10,000

5.0微克/毫克样本

1:1, 000-1:, 2000

Expiration date is one (1) year from date of opening.

IgG

MAPK3

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p44 MAP Kinase (ERK1) protein.

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

This affinity-purified antibody is directed against the human p44 MAP Kinase (ERK1) protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross reactivity with p44 MAP Kinase (ERK1) proteins from human, bovine, rabbit, rat and mouse. No reactivity is observed against p42 MAP Kinase (ERK2). Reactivity against homologues from other sources is not known.

小鼠

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ERK1 antibody, ERT 2 antibody, ERT2 antibody, Extracellular Signal Regulated Kinase 1 antibody, Extracellular signal related kinase 1 antibody, HGNC6877 antibody, HS44KDAP antibody, HUMKER1A antibody

人类

Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK’s) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively.  When activated, ERK’s form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the up-regulation of immediate early genes.  As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli.  Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.

多肽

No

No

C末端

酶联免疫吸附测定, 免疫沉淀, 免疫印迹

User Optimized

一抗

未共轭

P27361

91718899

抗p44地图激酶(ERK1)抗体-免疫印迹