抗体

NEDD4抗体

600-401-B05

100微克

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定

抗NEDD4(兔)抗体-600-401

NEDD4抗体

This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Nedd4 protein.   Specific conditions for reactivity and detection of Nedd4 should be optimized by the end user.  Expect a band approximately ~115 kDa in size corresponding to Nedd4 by western blotting in the appropriate cell lysate or extract.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

多克隆

0.64毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:20,000 – 1:35,000

User Optimized

1:300 to 1:500

Expiration date is one (1) year from date of opening.

IgG

NEDD4

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of the Nedd4 protein.

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous Nedd4 protein.  A BLAST analysis was used to suggest reactivity with Nedd4 from human, horse and macaque based on a 100% homology with the immunizing sequence.  Expect reactivity with Nedd4 from chimpanzee, rabbit, dog, and cattle based on a 94% homology with the immunizing sequence.  Cross-reactivity with Nedd4 from other sources has not been determined.

人类

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

rabbit anti-NEDD4 antibody, NEDD-4, NEDD 4, E3 ubiquitin protein ligase Nedd4 antibody, NEDD4-1 antibody, Neural precursor cell expressed developmentally down regulated 4 antibody, Cell proliferation-inducing gene 53 protein, HECT-type E3 ubiquitin transferase NEDD4, PIG53

人类

NEDD4 (neural precursor expressed, developmentally down-regulated protein 4, aliases: KIAA0093, MGC176705, NEDD4-1) is a gene that is highly expressed in early embryonic central nervous system. A family of NEDD4-like proteins has more recently been defined. NEDD4 and NEDD4-like proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4-2 have been shown to down-regulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations. The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins, and ubiquitination of these proteins, mediated by the HECT domain of NEDD4, results in their internalization and removal from the plasma membrane. Mutation of the PY motifs in ENaC proteins is associated with Liddle's Syndrome, an autosomal-dominant form of hypertension. In addition to targeting sodium channels, NEDD4-2 has also been shown to negatively regulate TGF-β signaling by targeting Smad2 for degradation. Mouse and human NEDD4 are cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear.

多肽

No

No

酶联免疫吸附测定, IF显微镜, 免疫印迹

User Optimized

一抗

未共轭

P46934

114520609

抗Nedd4抗体-免疫印迹