抗体

STAT3磷酸化Y705抗体

600-401-C64

100微克

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化

抗STAT3 pY705(兔)抗体-600-401

STAT3磷酸化Y705抗体

STAT3 pY705 antibody has been tested for use in ELISA, IHC, and Western Blot. Expect a band approximately 88.1 kDa in size corresponding to phosphorylated STAT3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

多克隆

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:10, 000 - 1:30, 000

User Optimized

1微克/毫升

Expiration date is one (1) year from date of opening.

IgG

STAT3

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This affinity purified Stat3 antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids surrounding tyrosine 705 of human Stat3.

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

Anti-STAT3 antibody is an affinity-purified antibody and is directed against the phosphorylated form of human STAT3 protein at the Y705. Anti-Stat3 antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Anti-Stat3 antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Stat3 protein is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with Stat3 antibodies from human, mouse, rat, and pig based on 100%sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

人类

Store vial at-20° C prior to opening. Aliquot contents and freeze at -20° C or below forextended storage. Avoid cycles of freezing and thawing. Centrifuge product ifnot completely clear after standing at room temperature. This product is stablefor several weeks at 4° C as an undiluted liquid. Dilute only prior toimmediate use.

rabbit anti-STAT3 pY705 antibody, Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, STAT3 phospho Y705, STAT3 (pTry705)

人类

Signal transducer and activator of transcription 3 (Stat3) belongs to a family of cytoplasmic transcription factors that can be activated by phosphorylation by its cell surface receptor. Stat3 plays a key role in many cellular processes such as cell growth and apoptosis. It also mediates cellular responses to interleukins, KITLG/SCF,EGF, IFN-alpha and other growth factors and may mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Stat3 forms a homodimer or a heterodimer with a related family member (e.g. STAT1). Activation occurs through phosphorylation of tyrosine 705 and serine 727. Phosphorylation of Stat3 at Tyr705 induces Stat3 dimerization and nuclear translocation. Serine phosphorylation is important for stable DNA-binding of Stat3 homodimers and maximal transcriptional activity. Stat3 can have a dual role in cancer, it has been found that Stat3 protein can promote oncogenesis and have a tumor suppressor role depending upon the mutational background of the tumor.

多肽

No

磷酸化

No

内部

酶联免疫吸附测定, 免疫组化, 免疫印迹

User Optimized

一抗

未共轭

P40763

NP_644805.1

免疫组化-STAT3 pY705抗体