产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
Collagen I alpha 1 propeptide Antibody
目录 :
600-401-D19
规格 :
100微升
价格 :
366.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 2
出版应用/物种/样本/稀释参考文献
  • 免疫组化-石蜡切片; 人类; 1:250
Rickelt S, Hynes R. Antibodies and methods for immunohistochemistry of extracellular matrix proteins. Matrix Biol. 2018;71-72:10-27 pubmed 出版商
Tian C, Huang Y, Clauser K, Rickelt S, Lau A, Carr S, et al. Suppression of pancreatic ductal adenocarcinoma growth and metastasis by fibrillar collagens produced selectively by tumor cells. Nat Commun. 2021;12:2328 pubmed 出版商
产品信息
目录号 :
600-401-D19
名称 :
Anti-Collagen I, alpha 1 propeptide (Rabbit) Antibody - 600-401-D19
显示名称 :
Collagen I alpha 1 propeptide Antibody
应用注释 :
Anti-Collagen I, alpha 1 propeptide Antibody is suitable for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 180 kDa in size corresponding to collagen I alpha I polypeptide in human lung fibroblast extracts or other appropriate tissues or lysates. Collagen I alpha 1 propeptide antibody also works well for IHC on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
Titrated value sufficient to run approximately 10 mini blots.
共轭 :
(None)
规格 :
100微升
默认单元 :
微升
ELISA稀释浓度 :
1:5000-1:50,000
免疫组化稀释度 :
1:100
免疫沉淀稀释度 :
1:100
免疫印迹稀释浓度 :
1:1000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG
基因名称 :
COL1A1
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-collagen I, alpha 1 propeptide Antibody was produced in rabbit by repeated immunizations with a synthetic peptide corresponding to amino acid residues specific to collagen 1, alpha 1 propeptide conjugated to KLH.
包装类型 :
干冰
物理状态 :
液体
纯度和特异性 :
Anti-Collagen I alpha 1 propeptide antibody is directed against collagen I alpha 1 propeptide (C-terminal region). Anti-Collagen I alpha 1 propeptide antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Anti-Collagen I alpha 1 propeptide antibody recognizes Collagen I alpha 1 propeptide from human, rat, and mouse sources. Cross reactivity with Collagen I alpha 1 propeptide from other species has not been determined. However, reactivity is also expected from most vertebrate species based on very high sequence homology.
物种活性 :
人类, 小鼠, 大鼠
储存 :
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
同义词 :
甲1Ⅰ型胶原, COLA1A1
靶物种 :
人类
背景 :
Collagen I alpha 1 propeptide Antibody detects collagen I which is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the must abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype. Anti-Collagen I alpha 1 propeptide Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
免疫原类型 :
多肽
低内毒素 :
No
样品尺寸 :
No
靶活性结构域 :
C末端
应用文本 :
酶联免疫吸附测定, 免疫组化, 免疫沉淀, 免疫印迹
类别 :
一抗
共轭名称 :
未共轭
2020价格 :
366.00美元
UniProt数据库 :
P02452
最初图像名称 :
Immunofluorescence Microscopy of Rabbit Anti-Collagen I-alpha 1-propeptide Antibody
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。