Anti-Ngp (neutrophilic granule protein) (RABBIT) Antibody - 600-401-GW9
This affinity purified antibody has been tested for use in IF, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band 39-42 kDa for non reduced and 20-27 kDa for reduced gels corresponding to Ngp by western blotting in the appropriate cell lysate or extract.
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
by UV absorbance at 280 nm
Expiration date is one (1) year from date of opening.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Affinity purified Anti-Ngp antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein of mouse Ngp protein.
Liquid (sterile filtered)
Anti-Ngp is directed against full-length mouse Ngp. This product is an affinity purified antibody produced by immunoaffinity chromatography using recombinant protein coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in Ngp based on 100% homology match for the immunogen sequence.
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
rabbit anti-Ngp antibody, Neutrophilic granule protein, Cystatin-like protein, Myeloid bactenecin protein, Myeloid secondary granule protein
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). A proteomic study in 2011 determined a direct correlation between NGP mRNA levels and myeloid-derived suppressor cells. Specifically cathepsin B, a catalytic mechanism and potentially biomarker in a variety of cancers and amyloid plaque development, was inhibited by NGP. The significance of NGP as a negative regulator of tumor metastasis makes this antibody ideal for investigators involved in cancer research, as well as Alzheimer’s research.
Western Blot of Rabbit anti-Ngp antibody
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Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部，为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立，利用In vitro及In vivo方法制备并纯化单抗及多抗，这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究：Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。