产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
组蛋白H3 K4me3抗体
目录 :
600-401-I59
规格 :
50微克
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫组化, 染色质免疫沉淀
更多信息或购买 :
产品信息
目录号 :
600-401-I59
名称 :
抗组蛋白H3[三甲基化Lys4](兔)抗体-600-401
显示名称 :
组蛋白H3 K4me3抗体
应用注释 :
Anti-Histone H3 [Trimethyl Lys4] antibody is useful for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
0.70毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
50微克
默认单元 :
微克
染色质免疫共沉淀稀释 :
2-5 µg/million cells
免疫组化稀释度 :
1:500
免疫荧光显微镜稀释浓度 :
1:500
免疫印迹稀释浓度 :
1:500
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG
基因名称 :
HIST2H3C
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Histone H3 [Trimethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 4 of human Histone H3.2.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
Anti-Histone H3 [Trimethyl Lys4] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
物种活性 :
Human, mouse, C. elegans
稳定剂 :
30% 甘油
储存 :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
兔抗组蛋白H3三甲基化Lys4抗体, H3.3B, H3.3AH3F3H3F3B, H3组蛋白, 家族3A, 组蛋白H3.3, MGC87783, MGC87782, H3K4me3
靶物种 :
人类
背景 :
H3K4Me3 is a common epigenetic modification that occurs in close proximity to nearly 2/3 of human gene promoters, and seems to balance another modification, H3K27Me3. These two modifications work in tandem to differentiate and/or maintain stem cells. Zygotic cells are activated in the most part by H3K4Me3; whereas, cancer is associated with disrupted associations between K3K4Me3 and ING1. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
免疫原类型 :
多肽
低内毒素 :
No
翻译后的修饰作用 :
甲基化
样品尺寸 :
No
靶活性结构域 :
N末端
应用文本 :
Chip,Immunohistochemistry,IF Microscopy,Western Blot,
其他 :
Dot Blot 1:1000
类别 :
一抗
共轭名称 :
未共轭
UniProt数据库 :
Q71DI3
NCBI :
NP_001005464
最初图像名称 :
组蛋白H3 K4/me3免疫荧光
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。