抗体

snail 1锌指蛋白抗体

600-401-MM5

100微克

多克隆

domestic rabbit

未共轭

人类, 小鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化

抗snail 1锌指蛋白(兔)抗体-600-401

snail 1锌指蛋白抗体

Anti-SNAI1 Antibody has been tested in WB, IHC, and IF. Expect a band at ~29 in western blot using appropriate lysates. Positive control used: SNAI1 overexpressed HEK293 in WB, human lung tissue in IHC, NIH-3T3 and HeLa in IF.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

多克隆

1.03毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:10,000-1:50,000

1:300

15µg/毫升

1:2000

Expiration date is one (1) year from date of opening.

IgG

snail 1锌指蛋白

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Anti-SNAI1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human SNAI1 conjugated to Keyhole Limpet Hemocyanin (KLH).

User Optimized

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

This affinity purified antibody is directed against human SNAI1.  This product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human and mouse.

人类, 小鼠

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Rabbit Anti-SNAI1 Antibody, Snail Family Transcriptional Repressor 1, Snail Family Zinc Finger 1, Zinc Finger Protein SNAI1, Protein Snail Homolog 1, Protein Sna, SNAH, Snail 1 (Drosophila Homolog), Zinc Finger Protein, Snail Homolog 1 (Drosophila), Snail 1 Zinc Finger Protein, Snail 1 Homolog, Snail Homolog 1, DJ710H13.1, SLUGH2, SNAIL1, SNAIL, SNA

人类

SNAI1 (Snail Family Transcriptional Repressor 1) is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. SNAI1 is involved in the induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. It binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. During EMT, SNAI1 is involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (By similarity). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). It associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, SNAI1 may also activate the CDKN2B promoter by itself. SNAI1 may play a role in Gastroesophageal Junction Adenocarcinoma and Fasciolopsiasis. Anti-SNAI1 Antibody is useful for researchers interested in Cytoskeleton remodeling Regulation of actin cytoskeleton by Rho GTPases and Kit receptor signaling pathways.

多肽

No

No

内部

酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫印迹

一抗

未共轭

O95863

NP_005976.2

Immunofluorescence of Rabbit Anti-SNAI1 Antibody