免疫印迹, 酶联免疫吸附测定, 免疫组化
图像 1 :
Immunofluorescence of Rabbit Anti-SNAI1 Antibody. Cell Line: NIH-3T3 cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-SNAI1 at 15µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Rabbit IgG DyLight™488 (p/n 611-741-127) at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). SNAI1 + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: nucleus, cytosol.
Anti-SNAI1 Antibody has been tested in WB, IHC, and IF. Expect a band at ~29 in western blot using appropriate lysates. Positive control used: SNAI1 overexpressed HEK293 in WB, human lung tissue in IHC, NIH-3T3 and HeLa in IF.
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
by UV absorbance at 280 nm
Expiration date is one (1) year from date of opening.
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Anti-SNAI1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human SNAI1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Liquid (sterile filtered)
This affinity purified antibody is directed against human SNAI1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human and mouse.
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Rabbit Anti-SNAI1 Antibody, Snail Family Transcriptional Repressor 1, Snail Family Zinc Finger 1, Zinc Finger Protein SNAI1, Protein Snail Homolog 1, Protein Sna, SNAH, Snail 1 (Drosophila Homolog), Zinc Finger Protein, Snail Homolog 1 (Drosophila), Snail 1 Zinc Finger Protein, Snail 1 Homolog, Snail Homolog 1, DJ710H13.1, SLUGH2, SNAIL1, SNAIL, SNA
SNAI1 (Snail Family Transcriptional Repressor 1) is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. SNAI1 is involved in the induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. It binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. During EMT, SNAI1 is involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (By similarity). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). It associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, SNAI1 may also activate the CDKN2B promoter by itself. SNAI1 may play a role in Gastroesophageal Junction Adenocarcinoma and Fasciolopsiasis. Anti-SNAI1 Antibody is useful for researchers interested in Cytoskeleton remodeling Regulation of actin cytoskeleton by Rho GTPases and Kit receptor signaling pathways.
酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫印迹
Immunofluorescence of Rabbit Anti-SNAI1 Antibody
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Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部，为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立，利用In vitro及In vivo方法制备并纯化单抗及多抗，这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究：Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。