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Immunofluorescence of Rabbit Anti-FGFR2 Antibody. Cells: U20S Cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-FGFR2 at 5µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Rabbit IgG DyLight™488 (p/n 611-741-127) at 5µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-FGFR2+DyLight™488. (C) Merge A+B. (D) Secondary Only. Expected localization: punctate cytoplasmic expression (Golgi), plasma membrane.
Anti-FGFR2 Antibody has been tested in ELISA, IF, and IHC. Positive control used: U-20S in immunofluorescence and mouse spleen and gallbladder in immunohistochemistry.
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
by UV absorbance at 280 nm
Expiration date is one (1) year from date of opening.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Anti-FGFR2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal portion of human FGFR2 conjugated to Keyhole Limpet Hemocyanin (KLH).
Liquid (sterile filtered)
This affinity purified antibody is directed against human FGFR2. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse.
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Rabbit Anti-Fibroblast Growth Factor Receptor 2 Antibody, Rabbit Anti-FGFR2 Antibody, Keratinocyte Growth Factor Receptor, Bacteria-Expressed Kinase, KGFR, BEK, Protein Tyrosine Kinase Receptor Like 14, BEK Fibroblast Growth Factor Receptor, Craniofacial Dysostosis 1, Jackson-Weiss Syndrome, Pfeiffer Syndrome, Crouzon Syndrome, CD332 Antigen, EC 220.127.116.11, FGFR-2, BFR-1, CD332, K-SAM, K-Sam, BBDS, CEK3, ECT1, TK14, TK25, CFD1, KSAM, JWS
FGFR2 (Fibroblast Growth Factor Receptor 2) are tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and are involved in a wide array of pathways known to play a significant role in cancer, angiogenesis, wound healing, cell migration, neural outgrowth and embryonic development. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. The mechanisms by which FGFR can be misregulated vary between cancers. Amplification of the receptors has been observed in lung and breast cancers, coding mutations and deletions have been seen in many cancers, and more recently, FGFR fusions that lead to pathway activation have been demonstrated to have oncogenic potential across multiple cancer types. Anti-FGFR2 Antibody is useful for researchers interested in lung cancer research, crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, bone and skin abnormalities, ERK Signaling, and Akt Signaling.
酶联免疫吸附测定, 免疫组化, IF显微镜
Immunofluorescence of Rabbit Anti-FGFR2 Antibody
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Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部，为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立，利用In vitro及In vivo方法制备并纯化单抗及多抗，这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究：Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。