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Immunofluorescence of Rabbit Anti-FGFR2 Antibody. Cells: U20S Cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-FGFR2 at 5µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Rabbit IgG DyLight™488 (p/n 611-741-127) at 5µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-FGFR2+DyLight™488. (C) Merge A+B. (D) Secondary Only. Expected localization: punctate cytoplasmic expression (Golgi), plasma membrane.
Anti-FGFR2 Antibody has been tested in ELISA, IF, and IHC. Positive control used: U-20S in immunofluorescence and mouse spleen and gallbladder in immunohistochemistry.
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
by UV absorbance at 280 nm
Expiration date is one (1) year from date of opening.
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Anti-FGFR2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal portion of human FGFR2 conjugated to Keyhole Limpet Hemocyanin (KLH).
Liquid (sterile filtered)
This affinity purified antibody is directed against human FGFR2. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse.
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Rabbit Anti-Fibroblast Growth Factor Receptor 2 Antibody, Rabbit Anti-FGFR2 Antibody, Keratinocyte Growth Factor Receptor, Bacteria-Expressed Kinase, KGFR, BEK, Protein Tyrosine Kinase Receptor Like 14, BEK Fibroblast Growth Factor Receptor, Craniofacial Dysostosis 1, Jackson-Weiss Syndrome, Pfeiffer Syndrome, Crouzon Syndrome, CD332 Antigen, EC 18.104.22.168, FGFR-2, BFR-1, CD332, K-SAM, K-Sam, BBDS, CEK3, ECT1, TK14, TK25, CFD1, KSAM, JWS
FGFR2 (Fibroblast Growth Factor Receptor 2) are tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and are involved in a wide array of pathways known to play a significant role in cancer, angiogenesis, wound healing, cell migration, neural outgrowth and embryonic development. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. The mechanisms by which FGFR can be misregulated vary between cancers. Amplification of the receptors has been observed in lung and breast cancers, coding mutations and deletions have been seen in many cancers, and more recently, FGFR fusions that lead to pathway activation have been demonstrated to have oncogenic potential across multiple cancer types. Anti-FGFR2 Antibody is useful for researchers interested in lung cancer research, crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, bone and skin abnormalities, ERK Signaling, and Akt Signaling.
酶联免疫吸附测定, 免疫组化, IF显微镜
Immunofluorescence of Rabbit Anti-FGFR2 Antibody
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