产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
Foxp3抗体
目录 :
600-401-MN0S
规格 :
25微升
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
Rockland Immunochemicals 600-401-MN0S 图像 1
Flow Cytometry of Rabbit Anti-Foxp3 Antibody. Cells: NIH-3T3 cells. Treatment: Fix/Perm solution for 30 minutes at 4°C and washed twice in Permeabilization Buffer. Primary Antibody: Anti-Foxp3 4µL in 100uL FACS buffer for 30 min at room temperature. Secondary Antibody: F(ab')2 Donkey Anti-Rabbit IgG Phycoerythrin (p/n 711-708-127) 5µL of secondary antibody in 100µL of reaction (used for for intra-nuclear staining). Isotype control: Rabbit Anti-Rad9 pS1129 Antibody (p/n 600-401-345).
产品信息
目录号 :
600-401-MN0S
名称 :
抗Foxp3(兔)抗体-600-401
显示名称 :
Foxp3抗体
应用注释 :
Anti-Foxp3 Antibody has been tested in ELISA, WB, IF, and FLOW.  Expect a band for isoforms 1 and 4 at ~47.2 and 44.4kDa in western blot using appropriate tissues and lysates.  Positive control used: NIH/3T3 cells in Immunofluorescence and Flow cytometry.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
0.99毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
25微升
默认单元 :
微升
ELISA稀释浓度 :
1:10,000-1:50,000
流式细胞仪稀释浓度 :
1:25
免疫荧光显微镜稀释浓度 :
15µg/毫升
免疫印迹稀释浓度 :
1:1000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG
基因名称 :
Foxp3
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-Foxp3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of mouse Foxp3 conjugated to Keyhole Limpet Hemocyanin (KLH).
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
This affinity purified antibody is directed against mouse Foxp3.  This product was affinity purified from monospecific antiserum by immunoaffinity purification.   A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse, human, and Macaca fascicularis.
物种活性 :
人类, 小鼠
储存 :
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
同义词 :
Rabbit Anti-Forkhead Box P3 Antibody, Immune Dysregulation Polyendocrinopathy Enteropathy, X-Linked, Forkhead Box Protein P3, Scurfin, IPEX, Immunodeficiency Polyendocrinopathy Enteropathy X-Linked, FOXP3delta7, DIETER, AIID, PIDX, XPID, JM2, Forkhead box protein P3 41 kDa form, Forkhead box protein P3 C-terminally processed
靶物种 :
小鼠
背景 :
FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 acts as a repressor of transcription and regulates T cell activation, with its overexpression in CD4 T cells leading to an attenuation of activation-induced cytokine production and proliferation. In regulatory T (Treg) cells, FOXP3 is essential for Treg suppressor function and its expression leads to the repression of IL-17 expression. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation. Anti-FOXP3 antibody is useful for researchers interested in transcription factor activity, polyendocrinopathy, immunodysregulation, and DNA binding research.
免疫原类型 :
多肽
低内毒素 :
No
样品尺寸 :
Yes
靶活性结构域 :
内部
应用文本 :
酶联免疫吸附测定, 流式细胞仪, IF显微镜, 免疫印迹
类别 :
一抗
共轭名称 :
未共轭
UniProt数据库 :
Q99JB6
NCBI :
NP_001186276.1
最初图像名称 :
Flow Cytometry of Rabbit Anti-Foxp3 Antibody
新产品 :
Yes
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。