公司名称 :
Rockland Immunochemicals
产品类型 :
产品名称 :
目录 :
规格 :
克隆性 :
宿主 :
domestic rabbit
共轭标签 :
反应物种 :
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪
更多信息或购买 :
图像 1 :
Rockland Immunochemicals 600-401-MN5S 图像 1
Flow Cytometry of Rabbit Anti-LC3B Antibody. Cells: U251-MG cells. Primary Antibody: Anti-LC3B at 2.5µg/mL in 100µL FACS buffer for 30 minutes at RT. Secondary Antibody: Donkey anti-Rabbit IgG DyLight™488 (p/n 611-711-127) at 2.5µg/mL in 100µL FACS buffer for 30 minutes at RT. Buffer: FACS/IF buffer (p/n MB-086-0500). Analysis: Distinct positive shifts in fluorescence have been observed in all samples tested following permeabilization of the cell sample. This is indicative of specificity and affinity of each antibody toward its target.
目录号 :
名称 :
显示名称 :
应用注释 :
Anti-LC3B Antibody has been tested in ELISA, Western Blot, Immunohistochemistry, and Flow Cytometry. Positive control used U251-MG Cells in Flow and Human lymph node in IHC. Testing only recommended in WB when using recombinant or overexpressing systems. Expect a band at ~14.6 kDa in western blot using appropriate lysates or tissues.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
浓度值 :
浓度定义 :
by UV absorbance at 280 nm
共轭 :
规格 :
默认单元 :
ELISA稀释浓度 :
流式细胞仪稀释浓度 :
免疫组化稀释度 :
免疫印迹稀释浓度 :
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
基因名称 :
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-LC3B antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human Microtubule-associated proteins 1A/1B light chain 3B conjugated to Keyhole Limpet Hemocyanin (KLH).
包装类型 :
物理状态 :
Liquid (sterile filtered)
防腐剂 :
纯度和特异性 :
This affinity purified antibody is directed against human LC3B.  This product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence.
物种活性 :
储存 :
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
同义词 :
Rabbit Anti-Microtubule Associated Protein 1 Light Chain 3 Beta Antibody, Rabbit Anti-LC3B Antibody, Microtubule-Associated Proteins 1A/1B Light Chain 3B, Autophagy-Related Ubiquitin-Like Modifier LC3 B, MAP1 Light Chain 3-Like Protein 2, MAP1A/MAP1B Light Chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-Associated Protein 1 Light Chain 3 Beta, Autophagy-Related Protein LC3 B, MAP1A/1BLC3, MAP1LC3B-A, MAP1ALC3, ATG8F, LC3B
靶物种 :
背景 :
MAP1LC3B (Microtubule Associated Protein 1 Light Chain 3 Beta) is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. It is a ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). LC3B plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Anti-LC3B Antibody is useful for researchers interested in spastic paraparesis, neuroendocrine system, neuroscience research, cancer research and apoptosis research.
免疫原类型 :
低内毒素 :
样品尺寸 :
靶活性结构域 :
应用文本 :
酶联免疫吸附测定, 流式细胞仪, 免疫组化, 免疫印迹
类别 :
共轭名称 :
UniProt数据库 :
最初图像名称 :
Flow Cytometry of Rabbit Anti-LC3B Antibody
更多信息或购买 :
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。