产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
蝶素b1, B2, B3抗体
目录 :
600-401-MP0S
规格 :
25微升
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 酶联免疫吸附测定
更多信息或购买 :
图像
图像 1 :
Rockland Immunochemicals 600-401-MP0S 图像 1
Immunofluorescence of Rabbit Anti-Ephrin B1-B3 Antibody. (A). Neural Plate of Xenopus laevis stage 17. (B). Knockdown neural plate of Xenopus laevis stage 17. Primary Antibody: Anti-Ephrin B1-3 at 1:5000 overnight at 2-8°C. Secondary Antibody: Anti-Rabbit IgG Fluorescent Secondary. Fixative: 4% Paraformaldehyde in PBS. Permeabilization: 0.05% Triton-X100 in PBS. Nuclear Counterstain: None. Magnification: 63X objective, Zeiss LSM710. Results: Ephrin B1 B2 B3 triple knockdown with a mixture of all three morpholinos abolished most of the signal which can be seen at expected plasma membrane and vesicle subcellular locations.
产品信息
目录号 :
600-401-MP0S
名称 :
抗蝶素b1, B2, B3(兔)抗体-600-401
显示名称 :
蝶素b1, B2, B3抗体
应用注释 :
Anti-Ephrin B1, B2, B3 Antibody has been tested in ELISA, Western Blot, and Immunofluorescence. Expect a band at ~35-38 kDa in western blot using appropriate lysates or tissues. Positive control used Xenopus laevis and mouse kidney in WB and Xenopus laevis in IF.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
1.05毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
25微升
默认单元 :
微升
ELISA稀释浓度 :
1:10, 000 - 50, 000
免疫荧光显微镜稀释浓度 :
1:5000
免疫印迹稀释浓度 :
1:1000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG
基因名称 :
EFNB1, Efnb2, EfnB3
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-Ephrin B1, B2, B3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-terminal portion of human Ephrin B1, B2, B3 conjugated to Keyhole Limpet Hemocyanin (KLH).
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
This affinity purified antibody is directed against human Ephrin B1, B2, B3.  This product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with Ephrin-B1 to human, mouse, rat, and Gallus gallus; 100% homology with Ephrin-B2 to mouse; 100% homology with Ephrin-B3 to human and mouse.
物种活性 :
人类, 小鼠, 非洲爪蛙
储存 :
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
同义词 :
Rabbit Anti-Ephrin B1, B2, B3 Antibody, Ephrin-B1, Ephrin-B2, Ephrin-B3, ELK Ligand, EPLG2, LERK2, EFL3, Craniofrontonasal Syndrome (Craniofrontonasal Dysplasia), EPH-Related Receptor Transmembrane Ligand ELK-L3, Eph-Related Receptor Tyrosine Kinase Ligand 2, Eph-Related Receptor Tyrosine Kinase Ligand 8, EPH-Related Receptor Tyrosine Kinase Ligand 2, LERK-2, LERK-8, Elk-L, EFL-3, ELK-L, CFND, EFL6, EFB1, CFNS
靶物种 :
人类
背景 :
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). Ephrin B1, B2, B3 are cell surface transmembrane ligands for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring. Ephrin B1 shows high affinity for the receptor tyrosine kinase EPHB1/ELK and can bind EPHB2 and EPHB3. Ephrin B3 may play a pivotal role in forebrain function. They binds to, and induces collapse of, commissural axons/growth cones in vitro. And may play a role in constraining the orientation of longitudinally projecting axons. Anti-Ephrin B1, B2, B3 Antibody is useful for researchers interested in Developmental Biology and Tyrosine Kinases/Adaptors.
免疫原类型 :
多肽
低内毒素 :
No
样品尺寸 :
Yes
靶活性结构域 :
C末端
应用文本 :
酶联免疫吸附测定, IF显微镜, 免疫印迹
类别 :
一抗
共轭名称 :
未共轭
UniProt数据库 :
P52800小鼠B2
NCBI :
NP_004420.1
最初图像名称 :
Immunofluorescence of Rabbit Anti-Ephrin B1/B3 Antibody
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。