产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
CD68抗体
目录 :
600-401-MP2S
规格 :
25微升
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
小鼠
应用 :
免疫印迹, 酶联免疫吸附测定
更多信息或购买 :
图像
图像 1 :
Rockland Immunochemicals 600-401-MP2S 图像 1
Western Blot of Rabbit Anti-CD68 Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HeLa + Rec. CD68 (Baculo expressed) protein (10µg/0.05µg) [+]. Lane 3: HeLa + Rec. CD68 (Baculo expressed) protein (10µg/0.02µg) [+]. Lane 4: HeLa + Rec. CD68 (Baculo expressed) protein (10µg/0.01µg) [+]. Lane 5: HeLa whole cell lysate (p/n W09-000-364) (10µg) [-]. Primary Antibody: Anti-CD68 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 60 mins at RT. Block: BlockOut Buffer (p/n MB-073). Expected MW: ~60-70kDa recombinant CD68. Observed MW: ~75kDa.
产品信息
目录号 :
600-401-MP2S
名称 :
抗CD68(兔)抗体-600-401
显示名称 :
CD68抗体
应用注释 :
Anti-CD68 Antibody has been tested in ELISA and Western Blot. Expect a band at ~34.8Da in western blot using appropriate tissues and lysates.  Positive control used: mouse heart and kidney in WB.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
1.02毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
25微升
默认单元 :
微升
ELISA稀释浓度 :
1:10,000-1:50,000
免疫印迹稀释浓度 :
1:1000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG
基因名称 :
CD68
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
CD68 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal region of mouse CD68.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
Anti-CD68 Antibody is directed against mouse CD68 protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence.  Cross reactivity with Cd68 from other sources has not been determined.
物种活性 :
小鼠
储存 :
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
同义词 :
Rabbit Anti-CD68 Antibody, Rabbit Anti-Macrosialin Antibody, Gp110, CD68, CD68 Molecule, CD68 Antigen, Scavenger Receptor Class D Member, Macrophage Antigen CD68, SCARD1, LAMP4, Macrosialin
靶物种 :
小鼠
背景 :
CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease. Anti-Cd68 antibody is suitable for researchers interested in cancer research and the immune system.
免疫原类型 :
多肽
低内毒素 :
No
样品尺寸 :
Yes
靶活性结构域 :
C末端
应用文本 :
酶联免疫吸附测定, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
UniProt数据库 :
P31996
NCBI :
NP_001277987.1
最初图像名称 :
Western Blot of Rabbit Anti-CD68 Antibody - rec detection
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。