产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
绿色荧光蛋白抗体荧光素共轭标签
目录 :
600-402-215
规格 :
100微克
价格 :
367.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
异硫氰酸荧光素
应用 :
免疫印迹, 酶联免疫吸附测定, 流式细胞仪
更多信息或购买 :
文章摘录数: 3
参考文献
Dolina J, Lee J, Griswold R, Labarta Bajo L, Kannan S, Greenbaum J, et al. TLR9 Sensing of Self-DNA Controls Cell-Mediated Immunity to Listeria Infection via Rapid Conversion of Conventional CD4+ T Cells to Treg. Cell Rep. 2020;31:107249 pubmed 出版商
Daniel S, Russ A, Guthridge K, Raina A, Estes P, Parsons L, et al. miR-9a mediates the role of Lethal giant larvae as an epithelial growth inhibitor in Drosophila. Biol Open. 2018;7: pubmed 出版商
Bankoti R, Ogawa C, Nguyen T, Emadi L, Couse M, Salehi S, et al. Differential regulation of Effector and Regulatory T cell function by Blimp1. Sci Rep. 2017;7:12078 pubmed 出版商
产品信息
目录号 :
600-402-215
名称 :
抗绿色荧光蛋白(兔)抗体荧光素共轭标签-600-402
显示名称 :
绿色荧光蛋白抗体荧光素共轭标签
应用注释 :
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
1.0毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
异硫氰酸荧光素
规格 :
100微克
默认单元 :
微克
ELISA稀释浓度 :
1:20,000-1:40,000
流式细胞仪稀释浓度 :
1:2000
荧光蛋白值 :
7.2
免疫荧光显微镜稀释浓度 :
1:500 - 1:2, 500
免疫印迹稀释浓度 :
1:10,000-1:25,000
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
包装类型 :
Ambient
物理状态 :
冻干
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
GFP Antibody Fluorescein Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum.  No reaction was observed against Human, Mouse and Rat Serum Proteins.
重组缓冲液 :
Restore with deionized water (or equivalent)
重组体积 :
100微升
物种活性 :
wt, rGFP, eGFP
稳定剂 :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
储存 :
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
同义词 :
rabbit anti-GFP antibody fluorescein conjugation, FITC conjugated rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
背景 :
Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
表位标签类型 :
绿色荧光蛋白
免疫原类型 :
重组蛋白
低内毒素 :
No
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, 流式细胞仪, IF显微镜, 免疫印迹
其他 :
FACS User optimized
类别 :
绿色荧光蛋白和表位
共轭名称 :
荧光素(异硫氰酸荧光素)
2020价格 :
367.00美元
最初图像名称 :
绿色荧光蛋白抗体荧光素共轭标签免疫印迹
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://rockland-inc.com
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。