抗体

III型胶原抗体生物素共轭标签

600-406-105

100微克

多克隆

domestic rabbit

生物素

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀

抗III型胶原(兔)抗体生物素共轭标签-600-406

III型胶原抗体生物素共轭标签

Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

多克隆

1.0毫克/毫升

by UV absorbance at 280 nm

BAC

100微克

微克

1:10, 000 - 1:50, 000

1:200 - 1:1, 000

1:100

1:1, 000 - 1:5, 000

Expiration date is one (1) year from date of opening.

IgG

COL3A1

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

III型胶原来自人类和牛胎盘

Ambient

冻干

0.01%(w/v)叠氮化钠

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.  Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards.  Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.   This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V and VI collagens.  Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Restore with deionized water (or equivalent)

100微升

人类, 牛

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Store vial at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

rabbit anti-Collagen Type III antibody biotin conjugation, biotin conjugated rabbit anti-Collagen Type III antibody, Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody, COL3A1, Collagen alpha-1 (III) chain

Mammalian

In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6% of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Type IV collagen is a major macromolecular constituent of basement membranes and can be readily isolated from basement-membrane-rich tissues or highly vascularized tissues such as the placental villi. This collagen appears to be largely restricted to structures identifiable as basement membranes. In contrast, type VI collagen appears to be prevalent in several tissues even though it has been isolated largely from placental villi preparations. The extent to which type VII and VIII collagens are distributed is not known.

天然蛋白

No

No

酶联免疫吸附测定, 免疫组化, 免疫沉淀, 免疫印迹

User Optimized

一抗

生物素

P02461

P02461.4

Rockland Anti-Collagen III Antibody - Immunohistochemistry