产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
绿色荧光蛋白抗体
目录 :
600-901-215
规格 :
100
价格 :
https://www.rockland.com/search/?searchString=600-901-215
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化
更多信息或购买 :
文章摘录数: 29
参考文献
Lamontagne J, Zhang H, Zeid A, Strittmatter K, Rocha A, Williams T, et al. Transcription factors AP-2α and AP-2β regulate distinct segments of the distal nephron in the mammalian kidney. Nat Commun. 2022;13:2226 pubmed 出版商
Ciesielski H, Nishida H, Takano T, Fukuhara A, Otani T, Ikegawa Y, et al. Erebosis, a new cell death mechanism during homeostatic turnover of gut enterocytes. PLoS Biol. 2022;20:e3001586 pubmed 出版商
Groschner L, Malis J, Zuidinga B, Borst A. A biophysical account of multiplication by a single neuron. Nature. 2022;603:119-123 pubmed 出版商
Zhao X, Fisher E, Wang Y, Zuloaga K, Manley L, Temple S. 4D imaging analysis of the aging mouse neural stem cell niche reveals a dramatic loss of progenitor cell dynamism regulated by the RHO-ROCK pathway. Stem Cell Reports. 2022;17:245-258 pubmed 出版商
Lyu C, Abbott L, Maimon G. Building an allocentric travelling direction signal via vector computation. Nature. 2022;601:92-97 pubmed 出版商
Osso L, Rankin K, Chan J. Experience-dependent myelination following stress is mediated by the neuropeptide dynorphin. Neuron. 2021;109:3619-3632.e5 pubmed 出版商
Li Y, Gong H, Zhan R, Ouyang S, Park K, Lutkenhaus J, et al. Genetic analysis of the septal peptidoglycan synthase FtsWI complex supports a conserved activation mechanism for SEDS-bPBP complexes. PLoS Genet. 2021;17:e1009366 pubmed 出版商
Wang Q, Pokhrel A, Coleman J. The Extracellular Superoxide Dismutase Sod5 From Fusarium oxysporum Is Localized in Response to External Stimuli and Contributes to Fungal Pathogenicity. Front Plant Sci. 2021;12:608861 pubmed 出版商
Oti T, Satoh K, Uta D, Nagafuchi J, Tateishi S, Ueda R, et al. Oxytocin Influences Male Sexual Activity via Non-synaptic Axonal Release in the Spinal Cord. Curr Biol. 2021;31:103-114.e5 pubmed 出版商
Sanders D, Kedersha N, Lee D, Strom A, Drake V, Riback J, et al. Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. Cell. 2020;181:306-324.e28 pubmed 出版商
Nam H, Capecchi M. Lrig1 expression prospectively identifies stem cells in the ventricular-subventricular zone that are neurogenic throughout adult life. Neural Dev. 2020;15:3 pubmed 出版商
Wu Y, Chawla G, SOKOL N. let-7-Complex MicroRNAs Regulate Broad-Z3, Which Together with Chinmo Maintains Adult Lineage Neurons in an Immature State. G3 (Bethesda). 2020;10:1393-1401 pubmed 出版商
Duan Z, Che A, Chu P, Mòdol L, Bollmann Y, Babij R, et al. GABAergic Restriction of Network Dynamics Regulates Interneuron Survival in the Developing Cortex. Neuron. 2019;: pubmed 出版商
Brito C, Mesquita F, Bleck C, Sellers J, Cabanes D, Sousa S. Perfringolysin O-Induced Plasma Membrane Pores Trigger Actomyosin Remodeling and Endoplasmic Reticulum Redistribution. Toxins (Basel). 2019;11: pubmed 出版商
Liu W, Zheng F, Wang Y, Fu C. Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope. J Mol Cell Biol. 2019;11:944-955 pubmed 出版商
Goswami M, Wang X, Zhang P, Xiao W, Karlen S, Li Y, et al. Novel window for cancer nanotheranostics: non-invasive ocular assessments of tumor growth and nanotherapeutic treatment efficacy in vivo. Biomed Opt Express. 2019;10:151-166 pubmed 出版商
Li M, Cao L, Xiao N, Tang M, Deng B, Yang T, et al. Hub-organized parallel circuits of central circadian pacemaker neurons for visual photoentrainment in Drosophila. Nat Commun. 2018;9:4247 pubmed 出版商
Fujiwara Y, Hermann Luibl C, Katsura M, Sekiguchi M, Ida T, Helfrich Forster C, et al. The CCHamide1 Neuropeptide Expressed in the Anterior Dorsal Neuron 1 Conveys a Circadian Signal to the Ventral Lateral Neurons in Drosophila melanogaster. Front Physiol. 2018;9:1276 pubmed 出版商
Ribeiro I, Drews M, Bahl A, Machacek C, Borst A, Dickson B. Visual Projection Neurons Mediating Directed Courtship in Drosophila. Cell. 2018;174:607-621.e18 pubmed 出版商
Oti T, Takanami K, Ito S, Ueda T, Matsuda K, Kawata M, et al. Effects of Sex Steroids on the Spinal Gastrin-Releasing Peptide System Controlling Male Sexual Function in Rats. Endocrinology. 2018;159:1886-1896 pubmed 出版商
Chiu C, Martenson J, Yamazaki M, Natsume R, Sakimura K, Tomita S, et al. Input-Specific NMDAR-Dependent Potentiation of Dendritic GABAergic Inhibition. Neuron. 2018;97:368-377.e3 pubmed 出版商
Kronenberg G, Uhlemann R, Richter N, Klempin F, Wegner S, Staerck L, et al. Distinguishing features of microglia- and monocyte-derived macrophages after stroke. Acta Neuropathol. 2018;135:551-568 pubmed 出版商
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Maruoka M, Kedashiro S, Ueda Y, Mizutani K, Takai Y. Nectin-4 co-stimulates the prolactin receptor by interacting with SOCS1 and inhibiting its activity on the JAK2-STAT5a signaling pathway. J Biol Chem. 2017;292:6895-6909 pubmed 出版商
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产品信息
目录号 :
600-901-215
名称 :
抗绿色荧光蛋白(鸡)抗体-600-901
显示名称 :
绿色荧光蛋白抗体
应用注释 :
Polyclonal anti-GFP is designed to detect GFP and its variants. Anti-GFP Antibody has been tested by ELISA, dot blot, and western and is suitable in immunofluorescence. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
多克隆
浓度值 :
1.17毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100
默认单元 :
微克
ELISA稀释浓度 :
1:10, 000 - 1:80, 000
免疫组化稀释度 :
1:200 - 1:1, 000
免疫荧光显微镜稀释浓度 :
User Optimized
免疫印迹稀释浓度 :
1:1, 000 - 1:5, 000
有效期 :
Expiration date is one (1) year from date of receipt.
格式 :
IgY
宿主动物 :
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
防腐剂 :
0.01%(w/v)叠氮化钠
纯度和特异性 :
Anti-GFP IgY was prepared from egg yolks by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).  No reaction was observed against Human, Mouse or Rat serum proteins.
物种活性 :
绿色荧光蛋白;eGFP;rGFP
检测过的应用 :
Dot Blot; ELISA; WB
Suggested Applications :
IF; IHC; Multiplex; Purification
储存 :
Store GFP Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
同义词 :
chicken anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
背景 :
Anti-GFP antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. GFP antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Chicken IgY lacks the classic "Fc" domain and does not bind to mammalian IgG Fc receptors. Thus resulting in lower backgrounds for western blotting, ELISA and Immunohistochemistry.
免疫原类型 :
重组蛋白
低内毒素 :
No
样品尺寸 :
No
应用文本 :
酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫印迹
其他 :
User Optimized
类别 :
一抗
共轭名称 :
未共轭
2024价格 :
$335.00
最初图像名称 :
抗绿色荧光蛋白抗体-免疫荧光显微镜
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。