抗体

GluR1磷酸化S845抗体

612-401-C83

150微升

多克隆

domestic rabbit

未共轭

小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化

抗GluR1 pS845(兔)抗体-612-401

GluR1磷酸化S845抗体

Anti-GluR1 pS845 antibody is suitable for use in ELISA, Western Blotting, IF and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 100 kDa in size corresponding to GluR1 protein phosphorylated at Ser 845 in Western Blots of rat brain extracts.

0.01 M羟乙基哌嗪乙磺酸, 0.15 M氯化钠, pH值7.5

多克隆

Titrated value sufficient to run approximately 10 mini blots.

(None)

150微升

微升

1:1000 - 10, 000

1:1000

1:200

1:1000

Expiration date is one (1) year from date of opening.

IgG

Gria1

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Anti-GluR1 pS845 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845.

干冰

液体

Anti-GluR1 pS845 Antibody is directed against GluR1 protein phophorylated at S845. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Minimal reactivity occurs against non-phosphorylated GluR1. Reactivity is completely eliminated by treatment with lambda-Phosphatase. Reactivity against GluR1 occurs from rat sources. However, reactivity is expected against the following species based on 100% sequence homology: human, mouse, and non-human primates.

小鼠, 大鼠

0.1毫克/毫升牛血清白蛋白(BSA)-不含IgG和蛋白酶, 50%(v/v)甘油

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Glutamate receptor 1, GluR1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1, Glutamate receptor ionotropic, AMPA 1

大鼠

Anti-GluR1 pS845 antibody recognizes phophorylated GluR1. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR. In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory. Anti-GluR1 pS845 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

多肽

No

磷酸化

No

内部

酶联免疫吸附测定, 免疫组化, IF显微镜, 免疫印迹

一抗

未共轭

P19490

NP_113796.1

Western blot of GluR1-Subunit Ser845 Antibody