抗体

连接蛋白磷酸化S368抗体

612-401-D23

100微升

多克隆

domestic rabbit

未共轭

小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定

抗连接蛋白pS368(兔)抗体-612-401

连接蛋白磷酸化S368抗体

Anti-Connexin 43 pS368 Antibody is suitable for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 43 kDa in size corresponding to connexin 43 protein phosphorylated at Ser368 in the appropriate cell lysate or extract.

0.01 M羟乙基哌嗪乙磺酸, 0.15 M氯化钠, pH值7.5

多克隆

Titrated value sufficient to run approximately 10 mini blots.

(None)

100微升

微升

1:10,000

1:1000

Expiration date is one (1) year from date of opening.

IgG

Gja1

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Anti-Connexin 43 pS368 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to KLH.

干冰

液体

Anti-Connexin 43 pS368 antibody is directed against rat pS368 Connexin 43. Anti-Connexin 43 pS368 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated Connexin 43. Minimal reactivity occurs against non-phosphorylated Connexin 43. Reactivity is completely blocked by treatment with lambda-phosphatase. Reactivity against Connexin 43 occurs from rat sources. However, reactivity is also expected against bovine, canine, chicken, guinea pig, human, mouse and zebra fish sources.

小鼠, 大鼠

0.1毫克/毫升牛血清白蛋白(BSA)-不含IgG和蛋白酶, 50%(v/v)甘油

Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Gap junction alpha-1 protein, Gap junction 43 kDa heart protein, CX43, Connexin-43

大鼠

Anti-Connexin 43 pS368 Antibody detects pS368 Connexin which is thought to play a key role in development and may also be involved in epilepsy. Connexin 43 antibody recognizes Connexin43 which forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C. Phosphorylation of Ser368 by PKC induces a conformational change of connexin 43 that results in a decrease in gap junction permeability. Anti-Connexin 43 pS368 Antibody is ideal for investigators involved in Neuroscience and Cell Signaling.

多肽

No

磷酸化

No

内部

酶联免疫吸附测定, 免疫印迹

一抗

未共轭

P08050

AAA41000.1

Western Blot of Anti-Connexin 43 pS368 (Rabbit) Antibody - 612-401-D23