血管内皮生长因子抗体过氧化物酶 共轭标签
Rockland Immunochemicals
目录: 209-403-B99
domestic rabbit 多克隆
反应物种: 人类
共轭标签: HRP
应用: 免疫印迹, 免疫组化

Western Blot showing detection of Recombinant Human VEGF-165. 50ng of protein (Lane 1) was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C, Anti-VEGF-165 (RABBIT) Antibody Peroxidase Conjugate (p/n 209-403-B99) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP. Arrow indicates correct 19 kDa molecular weight position expected for rH-VEGF-165.
规格: 100微克
价格:
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血管内皮生长因子-165抗体
Rockland Immunochemicals
目录: 209-401-B99
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定

Rockland's anti-Human VEGF-165 antibody shows detection of a band ~22 kDa in size corresponding to recombinant human VEGF-165. This isoform of VEGF tends to form a disulfide linked homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
规格: 100微克
价格:
至产商
血管内皮生长因子-121抗体
Rockland Immunochemicals
目录: 209-401-C23
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定

Anti-human VEGF-121 by western blot shows detection of recombinant Human VEGF-121 raised in E.coli. Recombinant (0.1 µg, 28.4 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Rockland's, Inc. Anti-Human VEGF-121. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
规格: 100微克
价格:
至产商
血管内皮生长因子抗体生物素共轭标签
Rockland Immunochemicals
目录: 209-406-B99
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
规格: 100微克
价格:
至产商
Recombinant Anti-VEGF Fab Antibody
Rockland Immunochemicals
目录: 400-001-MT4
重组 (Lucentis)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪

Western Blot using Anti-human VEGF Fab fragment Antibody to detect human VEGF165 protein. MW: Molecular Weight Ladder. Lanes 1, 3, and 5: VEGF165 reduced. Lanes 2, 4, and 6: VEGF165 non-reduced. Protein Load: Lane 1-2: 200ng. Lane 3-4: 40ng. Lane 5-6: 8ng. Primary Antibody: Anti-VEGF Fab fragment at 1.0µg/mL for 2 hours at 4°C. Secondary Antibody: F(ab')2 Human IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed (p/n 709-1318) at 1:5,000 dilution was used. The VEGF165 protein has a calculated MW of 19.2kDa. As a result of glycosylation, the protein migrates as 24kDa (monomer) under reducing (R) condition, and 43-50kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).
规格: 50
价格: 465.00美元
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Recombinant Anti-VEGF RTH258 scFv Antibody
Rockland Immunochemicals
目录: 400-001-MT5
重组 (Abz5)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪

Western Blot using Humanized Recombinant Brolucizumab/RTH258 Anti-human VEGF ScFv to detect structured and denatured human VEGF165 protein. MW: Molecular Weight Ladder. Lanes 1,3, and 5: VEGF165 reduced. Lanes 2, 4, and 6: VEGF165 non-reduced. Protein Load: Lane 1-2: 200ng. Lane 3-4: 40ng. Lane 5-6: 8ng. Primary Antibody: FLAG-tagged Humanized Recombinant Brolucizumab/RTH258 Anti-human VEGF ScFv at 1.0µg/mL for 2 hours at 4°C. Secondary Antibody: Antibody for the detection of FLAG™ conjugated proteins (MOUSE) Monoclonal Antibody Peroxidase Conjugated (p/n 200-303-383) at 1:5000 for 2 hours at 4°C. The VEGF165 protein has a calculated MW of 19.2kDa. As a result of glycosylation, the protein migrates as 24kDa (monomer) under reducing (R) condition, and 43-50kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).
规格: 50
价格: 465.00美元
至产商