二抗

Rabbit TrueBlot® Set (IP Agarose beads)

88-1688-31

1套

243.00美元

Rabbit TrueBlot® Set (with IP Agarose beads) - 88-1688-31

Rabbit TrueBlot® Set (IP Agarose beads)

Rabbit IgG TrueBlot® is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 20 blots. Note that there are three key procedural considerations: 1. Protein A or G should not be used for the immunoprecipitation. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. For immunoprecipitation, Anti-Rat IgG beads, or Anti-Rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. Rabbit TrueBlot Set Components: 1. Rabbit IgG TrueBlot® HRP-conjugated monoclonal secondary antibody reacting with rabbit IgGs for optimal signal detection in immunoprecipitation/immunoblot experiments. 2. Anti-Rabbit Ig IP Beads: 2.5 mL. Binds 1 mg Ig/mL beads. 3. Western blot incubation tray. Special Notes: Upon initial use of the IP beads, we recommend that the vial be inverted several times to get the beads into suspension. We recommend using a large bore pipet to pipet up the liquid for use. For storage of the opened vial of beads, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user.

0.01 M钠磷酸盐, 0.15 M氯化钠, pH值7.2

单克隆

eB182

HRP ULTRA套

1套

套

TrueBlot anti-Rabbit Ig IP Beads (binds 2.5 mg Ig/mL beads) have been reported for use in IP

1:1000

Expiration date is six (6) months from date of opening.

IgG

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Wet Ice

Liquid (sterile filtered)

Rabbit TrueBlot® Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against Anti-Rabbit Serum. Reactivity is observed against native Rabbit IgG by both Western blot and ELISA.

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0.1毫克/毫升牛血清白蛋白(BSA)-不含IgG和蛋白酶, 50%(v/v)甘油

Store TrueBlot® Anti-Rabbit Ig IP beads (00-8800-25) at 2-8 °C and Rabbit TrueBlot® (18-8816-31) at -20 °C. This product is guaranteed for 6 months upon receipt, when handled and stored as instructed.

Anti-Rabbit Immunoglobulin Gamma, Agarose-conjugated IgG, Gt-a-Rb IgG, Goat-anti-Rabbit IgG, TrueBlot for immunoprecipitation, IP Agarose beads for TrueBlot, HRP, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for western blotting

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Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated Anti-Rabbit IgG monoclonal secondary antibody. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Rabbit IgG TrueBlot®, simply substitute the conventional HRP Anti-Rabbit IgG blotting reagent with Rabbit IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Rabbit IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Rabbit IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation.

Immunoprecipitation Kit

Kit

No

No

免疫沉淀, 免疫印迹

Kits

HRP TrueBlot® ULTRA for IP/WB (with IP beads)

243.00美元

Rabbit TrueBlot® IP / Western Blot