抗体

Ki67抗原抗体

900-C01-B38

100微升

单克隆

domestic rabbit

未共轭

SP6

人类, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化

抗Ki67抗原(兔)单克隆抗体-900-C01

Ki67抗原抗体

This monoclonal antibody contains TBS/1% BSA and is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 345 and 395 kDa in size corresponding to Ki-67 protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min. Ki-67 is a nuclear protein. Tonsil tissue can be used as a positive control.

See application note.

单克隆

SP6

(None)

100微升

微升

1:10,000

1:200

1:200-1:500

Expiration date is one (1) year from date of opening.

CCS

MKI67

兔

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminus of human Ki-67 protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.

Wet Ice

Liquid (sterile filtered)

0.1%(w/v)叠氮化钠

This product is clarified tissue culture supernatant. The antibody is specific for human Ki-67 protein. Cross-reactivity occurs with rat Ki-67. Cross reactivity with Ki-67 protein from other sources has not been determined.

人类, 大鼠

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date.

IgG

兔抗Ki67抗原抗体, MKI67, Ki67抗原核蛋白质

人类

The MKI67 gene encodes a nuclear protein termed Ki-67 that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. Two isoforms have been reported, with isoform 1 transcribed as the longer transcript. Ki-67 is a nuclear protein predominantly localized in the G1 phase in the perinucleolar region. In later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.

多肽

No

No

C末端

酶联免疫吸附测定, 免疫组化, 免疫印迹

User Optimized

一抗

未共轭

P46013

CAI16903.1

抗Ki67抗原单克隆抗体-免疫组化