产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
其他
产品名称 :
Proteinase K (Tritirachium album limber)
目录 :
MB-112-0100
规格 :
100毫克
更多信息或购买 :
产品信息
目录号 :
MB-112-0100
名称 :
Proteinase K (Tritirachium album limber) - MB-112-0100
显示名称 :
Proteinase K (Tritirachium album limber)
应用注释 :
Proteinase K is specifically formulated for molecular cloning, nucleic acid research and protein/peptide structural analysis. One unit liberates one µmole of Folin positive amino acids, measured as tyrosine, at 37° C at pH 7.5 using urea denatured hemoglobin as the substrate. Recommended working concentration 0.05-0.1mg/mL.
共轭 :
(None)
规格 :
100毫克
默认单元 :
毫克
有效期 :
Expiration date is one (1) year from date of opening.
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
其他性能数据 :
A280 at 1 mg/ml = 0.65 % Ribonuclease: None detected % Deoxyribonuclease: None detected
包装类型 :
Ambient
物理状态 :
冻干
纯度和特异性 :
A lyophilized powder. Chromatographically purified to remove DNase and RNase activity. Stable over a wide pH range : 4.0-12.5, optimal pH 7.5-8.0, using denatured hemaglobin as substrate. Although calcium ions do not affect the enzyme activity, they do protect Proteinase K against autolysis and increase thermal stability when present at a concentration of 1-5µmoles. It retains its activity in the presence of SDS or urea (0.5-1% SDS and 1-4M urea). Raising the tempurature of the reaction from 37°C to 50-60°C can increase the activity several fold. Proteinase K is inactivated by diisopropyl fluorophosphate or phenyl methane sulphonyl fluoride. Chelating agents such as citrate and EDTA have no effect on the enzyme activity. Proteinase K can also be inactivated by heating above 65°C for 15-20 min or by extraction with phenyl/chloroform.
特定活性值 :
31.4单位/毫克
特定活性定义 :
by dry weight
储存 :
Store vial at 2-8°C prior to restoration. Solutions in 50mM Tris-HCl, pH 8.0, 1mM CaCl2 stored at 2-8°C are stable for months.
同义词 :
蛋白酶K, 内肽酶K
背景 :
Proteinase K is a broad-spectrum serine protease and is able to digest native keratin (hair), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.
低内毒素 :
No
MoBio酶比较DNA比较分子量标记 :
MoBio溶液比较试剂 :
试剂
样品尺寸 :
No
类别 :
分子生物学试剂
共轭名称 :
未共轭
更多信息或购买 :
公司信息
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/
800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。