产品简要
公司名称 :
西格玛奥德里奇
其他品牌 :
FLUKA
产品类型 :
抗体
产品名称 :
Monoclonal Anti-alpha-Actinin (Sarcomeric) antibody produced in mouse
目录 :
A7732
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
EA-53
反应物种 :
人类, 小鼠, 大鼠, , , ,
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片, immunohistochemistry - free floating section, 其他
更多信息或购买 :
文章摘录数: 50
出版应用/物种/样品/稀释参考文献
  • 免疫组化-冰冻切片; 小鼠; 1:200; 图 2a
Pappas C, Farman G, Mayfield R, Konhilas J, Gregorio C. Cardiac-specific knockout of Lmod2 results in a severe reduction in myofilament force production and rapid cardiac failure. J Mol Cell Cardiol. 2018;122:88-97 pubmed 出版商
  • 免疫组化; 小鼠; 1:100
  • 免疫印迹; 小鼠; 1:5000; 图 3d
  • 免疫印迹; 人类; 1:5000; 图 1b
Reinhard J, Lin S, McKee K, Meinen S, Crosson S, Sury M, et al. Linker proteins restore basement membrane and correct LAMA2-related muscular dystrophy in mice. Sci Transl Med. 2017;9: pubmed 出版商
  • 免疫细胞化学; 人类; 1:1000; 图 3a
Jeziorowska D, Fontaine V, Jouve C, Villard E, Dussaud S, Akbar D, et al. Differential Sarcomere and Electrophysiological Maturation of Human iPSC-Derived Cardiac Myocytes in Monolayer vs. Aggregation-Based Differentiation Protocols. Int J Mol Sci. 2017;18: pubmed 出版商
  • 免疫印迹; 大鼠; 1:5000; 图 5b
Kovacs A, Kalász J, Pasztor E, Toth A, Papp Z, Dhalla N, et al. Myosin heavy chain and cardiac troponin T damage is associated with impaired myofibrillar ATPase activity contributing to sarcomeric dysfunction in Ca2+-paradox rat hearts. Mol Cell Biochem. 2017;430:57-68 pubmed 出版商
  • 免疫细胞化学; 大鼠; 1:500; 图 5a
Fukuda R, Gunawan F, Beisaw A, Jiménez Amilburu V, Maischein H, Kostin S, et al. Proteolysis regulates cardiomyocyte maturation and tissue integration. Nat Commun. 2017;8:14495 pubmed 出版商
  • 流式细胞仪; 人类; 1:800; 图 1d
Kempf H, Olmer R, Haase A, Franke A, Bolesani E, Schwanke K, et al. Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells. Nat Commun. 2016;7:13602 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:200; 图 5a
Su F, Myers V, Knezevic T, Wang J, Gao E, Madesh M, et al. Bcl-2-associated athanogene 3 protects the heart from ischemia/reperfusion injury. JCI Insight. 2016;1:e90931 pubmed
  • 免疫印迹; 人类; 1:2500; 图 4b
O Grady G, Best H, Sztal T, Schartner V, Sanjuan Vazquez M, Donkervoort S, et al. Variants in the Oxidoreductase PYROXD1 Cause Early-Onset Myopathy with Internalized Nuclei and Myofibrillar Disorganization. Am J Hum Genet. 2016;99:1086-1105 pubmed 出版商
  • 免疫印迹; 人类; 1:2000; 图 10
Hammers D, Sleeper M, Forbes S, Shima A, Walter G, Sweeney H. Tadalafil Treatment Delays the Onset of Cardiomyopathy in Dystrophin-Deficient Hearts. J Am Heart Assoc. 2016;5: pubmed 出版商
  • 免疫细胞化学; 人类; 图 4k
Suchanek J, Suchánková Kleplová T, Rehacek V, Browne K, Soukup T. Proliferative Capacity and Phenotypical Alteration of Multipotent Ecto-Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth Cultured in Xenogeneic and Allogeneic Media. Folia Biol (Praha). 2016;62:1-14 pubmed
  • 免疫细胞化学; 小鼠; 1:200; 图 2d
Tang Y, Hong Y, Bai H, Wu Q, Chen C, Lang J, et al. Plant Homeo Domain Finger Protein 8 Regulates Mesodermal and Cardiac Differentiation of Embryonic Stem Cells Through Mediating the Histone Demethylation of pmaip1. Stem Cells. 2016;34:1527-40 pubmed 出版商
  • 其他; 小鼠; 1:800; 图 2a
Saito Y, Nakamura K, Yoshida M, Sugiyama H, Ohe T, Kurokawa J, et al. Enhancement of Spontaneous Activity by HCN4 Overexpression in Mouse Embryonic Stem Cell-Derived Cardiomyocytes - A Possible Biological Pacemaker. PLoS ONE. 2015;10:e0138193 pubmed 出版商
  • 免疫细胞化学; 小鼠
Yang J, Kaur K, Ong L, Eisenberg C, Eisenberg L. Inhibition of G9a Histone Methyltransferase Converts Bone Marrow Mesenchymal Stem Cells to Cardiac Competent Progenitors. Stem Cells Int. 2015;2015:270428 pubmed 出版商
  • 免疫细胞化学; 人类; 1:800; 图 6
Palpant N, Hofsteen P, Pabon L, Reinecke H, Murry C. Cardiac development in zebrafish and human embryonic stem cells is inhibited by exposure to tobacco cigarettes and e-cigarettes. PLoS ONE. 2015;10:e0126259 pubmed 出版商
  • 免疫印迹; 人类
Kalinowska M, Chávez A, Lutzu S, Castillo P, Bukauskas F, Francesconi A. Actinin-4 Governs Dendritic Spine Dynamics and Promotes Their Remodeling by Metabotropic Glutamate Receptors. J Biol Chem. 2015;290:15909-20 pubmed 出版商
  • 免疫组化; 人类; 1:200; 图 4
Tchao J, Han L, Lin B, Yang L, Tobita K. Combined biophysical and soluble factor modulation induces cardiomyocyte differentiation from human muscle derived stem cells. Sci Rep. 2014;4:6614 pubmed 出版商
  • 免疫细胞化学; 大鼠
Bingen B, Engels M, Schalij M, Jangsangthong W, Neshati Z, Feola I, et al. Light-induced termination of spiral wave arrhythmias by optogenetic engineering of atrial cardiomyocytes. Cardiovasc Res. 2014;104:194-205 pubmed 出版商
  • 免疫组化-冰冻切片; 小鼠
Can T, Faas L, Ashford D, Dowle A, Thomas J, O Toole P, et al. Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections. Proteome Sci. 2014;12:25 pubmed 出版商
  • 免疫组化; 人类
Janbaz A, Lindström M, Liu J, Pedrosa Domellöf F. Intermediate filaments in the human extraocular muscles. Invest Ophthalmol Vis Sci. 2014;55:5151-9 pubmed 出版商
  • 免疫印迹; 大鼠
Corpeno R, Dworkin B, Cacciani N, Salah H, Bergman H, Ravara B, et al. Time course analysis of mechanical ventilation-induced diaphragm contractile muscle dysfunction in the rat. J Physiol. 2014;592:3859-80 pubmed 出版商
  • 免疫组化; 小鼠; 图 4e
Kudo Sakamoto Y, Akazawa H, Ito K, Takano J, Yano M, Yabumoto C, et al. Calpain-dependent cleavage of N-cadherin is involved in the progression of post-myocardial infarction remodeling. J Biol Chem. 2014;289:19408-19 pubmed 出版商
  • immunohistochemistry - free floating section; 大鼠; 10 ug/ml
King A, Manning C, Trimmer J. A unique ion channel clustering domain on the axon initial segment of mammalian neurons. J Comp Neurol. 2014;522:2594-608 pubmed 出版商
  • 免疫印迹; 小鼠; 1:750
Roehrich M, Spicher A, Milano G, Vassalli G. Characterization of cardiac-resident progenitor cells expressing high aldehyde dehydrogenase activity. Biomed Res Int. 2013;2013:503047 pubmed 出版商
  • 免疫组化-石蜡切片; 小鼠; 1:2000
Willaredt M, Gorgas K, Gardner H, Tucker K. Multiple essential roles for primary cilia in heart development. Cilia. 2012;1:23 pubmed 出版商
Chai S, Wan X, Ramirez Navarro A, Tesar P, Kaufman E, Ficker E, et al. Physiological genomics identifies genetic modifiers of long QT syndrome type 2 severity. J Clin Invest. 2018;128:1043-1056 pubmed 出版商
Long C, Li H, Tiburcy M, Rodriguez Caycedo C, Kyrychenko V, Zhou H, et al. Correction of diverse muscular dystrophy mutations in human engineered heart muscle by single-site genome editing. Sci Adv. 2018;4:eaap9004 pubmed 出版商
Sassi Y, Avramopoulos P, Ramanujam D, Grüter L, Werfel S, Giosele S, et al. Cardiac myocyte miR-29 promotes pathological remodeling of the heart by activating Wnt signaling. Nat Commun. 2017;8:1614 pubmed 出版商
Cowling B, Prokic I, Tasfaout H, Rabai A, Humbert F, Rinaldi B, et al. Amphiphysin (BIN1) negatively regulates dynamin 2 for normal muscle maturation. J Clin Invest. 2017;: pubmed 出版商
Judge L, Perez Bermejo J, Truong A, Ribeiro A, Yoo J, Jensen C, et al. A BAG3 chaperone complex maintains cardiomyocyte function during proteotoxic stress. JCI Insight. 2017;2: pubmed 出版商
Fernandez Perez A, Munshi N. Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts. J Vis Exp. 2017;: pubmed 出版商
Chao M, Gentles A, Chatterjee S, Lan F, Reinisch A, Corces M, et al. Human AML-iPSCs Reacquire Leukemic Properties after Differentiation and Model Clonal Variation of Disease. Cell Stem Cell. 2017;20:329-344.e7 pubmed 出版商
Tsukamoto S, Fujii T, Oyama K, Shintani S, Shimozawa T, Kobirumaki Shimozawa F, et al. Simultaneous imaging of local calcium and single sarcomere length in rat neonatal cardiomyocytes using yellow Cameleon-Nano140. J Gen Physiol. 2016;148:341-55 pubmed 出版商
Lindqvist J, Levy Y, Pati Alam A, Hardeman E, Gregorevic P, Ochala J. Modulating myosin restores muscle function in a mouse model of nemaline myopathy. Ann Neurol. 2016;79:717-725 pubmed 出版商
Mishra S, Chatterjee S. Lactosylceramide promotes hypertrophy through ROS generation and activation of ERK1/2 in cardiomyocytes. Glycobiology. 2014;24:518-31 pubmed 出版商
San Antonio A, Liban K, Ikrar T, Tsyganovskiy E, Xu X. Distinct physiological and developmental properties of hippocampal CA2 subfield revealed by using anti-Purkinje cell protein 4 (PCP4) immunostaining. J Comp Neurol. 2014;522:1333-54 pubmed 出版商
Christoforou N, Liau B, Chakraborty S, Chellapan M, Bursac N, Leong K. Induced pluripotent stem cell-derived cardiac progenitors differentiate to cardiomyocytes and form biosynthetic tissues. PLoS ONE. 2013;8:e65963 pubmed 出版商
Caruso N, Herberth B, Bartoli M, Puppo F, Dumonceaux J, Zimmermann A, et al. Deregulation of the protocadherin gene FAT1 alters muscle shapes: implications for the pathogenesis of facioscapulohumeral dystrophy. PLoS Genet. 2013;9:e1003550 pubmed 出版商
Drawnel F, Wachten D, Molkentin J, Maillet M, Aronsen J, Swift F, et al. Mutual antagonism between IP(3)RII and miRNA-133a regulates calcium signals and cardiac hypertrophy. J Cell Biol. 2012;199:783-98 pubmed 出版商
Ritner C, Wong S, King F, Mihardja S, Liszewski W, Erle D, et al. An engineered cardiac reporter cell line identifies human embryonic stem cell-derived myocardial precursors. PLoS ONE. 2011;6:e16004 pubmed 出版商
Bang M, Li X, Littlefield R, Bremner S, Thor A, Knowlton K, et al. Nebulin-deficient mice exhibit shorter thin filament lengths and reduced contractile function in skeletal muscle. J Cell Biol. 2006;173:905-16 pubmed
Faul C, Huttelmaier S, Oh J, Hachet V, Singer R, Mundel P. Promotion of importin alpha-mediated nuclear import by the phosphorylation-dependent binding of cargo protein to 14-3-3. J Cell Biol. 2005;169:415-24 pubmed
Mudry R, Perry C, Richards M, Fowler V, Gregorio C. The interaction of tropomodulin with tropomyosin stabilizes thin filaments in cardiac myocytes. J Cell Biol. 2003;162:1057-68 pubmed
McElhinny A, Kakinuma K, Sorimachi H, Labeit S, Gregorio C. Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1. J Cell Biol. 2002;157:125-36 pubmed
Braz J, Bueno O, De Windt L, Molkentin J. PKC alpha regulates the hypertrophic growth of cardiomyocytes through extracellular signal-regulated kinase1/2 (ERK1/2). J Cell Biol. 2002;156:905-19 pubmed
van der Flier A, Kuikman I, Kramer D, Geerts D, Kreft M, Takafuta T, et al. Different splice variants of filamin-B affect myogenesis, subcellular distribution, and determine binding to integrin [beta] subunits. J Cell Biol. 2002;156:361-76 pubmed
Palmer S, Groves N, Schindeler A, Yeoh T, Biben C, Wang C, et al. The small muscle-specific protein Csl modifies cell shape and promotes myocyte fusion in an insulin-like growth factor 1-dependent manner. J Cell Biol. 2001;153:985-98 pubmed
Ehler E, Horowits R, Zuppinger C, Price R, Perriard E, Leu M, et al. Alterations at the intercalated disk associated with the absence of muscle LIM protein. J Cell Biol. 2001;153:763-72 pubmed
Bang M, Mudry R, McElhinny A, Trombitas K, Geach A, Yamasaki R, et al. Myopalladin, a novel 145-kilodalton sarcomeric protein with multiple roles in Z-disc and I-band protein assemblies. J Cell Biol. 2001;153:413-27 pubmed
Scholl F, McLoughlin P, Ehler E, De Giovanni C, Schafer B. DRAL is a p53-responsive gene whose four and a half LIM domain protein product induces apoptosis. J Cell Biol. 2000;151:495-506 pubmed
van der Ven P, Wiesner S, Salmikangas P, Auerbach D, Himmel M, Kempa S, et al. Indications for a novel muscular dystrophy pathway. gamma-filamin, the muscle-specific filamin isoform, interacts with myotilin. J Cell Biol. 2000;151:235-48 pubmed
产品信息
目录号 :
A7732
品牌 :
SIGMA
名称 :
Monoclonal Anti-alpha-Actinin (Sarcomeric) antibody produced in mouse
产品类型 :
化学品
名字后缀 :
~1.0 mg/mL, clone EA-53, purified immunoglobulin, buffered aqueous solution
储存 :
-20C
储存温度 :
-20C
物理形态 :
溶液在0.01 M磷酸盐缓冲液, pH值7.4, 含15 mM叠氮化钠
运输的 :
干冰
同义词 :
抗1110008F24Rik;抗ACTN2;抗辅肌动蛋白alpha2;抗CMD1AA;抗MGC107582
MDL号 :
MFCD00164521
形式 :
buffered aqueous solution
抗体形态 :
纯化免疫球蛋白
应用(s) :
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 mug/mL using protease-digested sections of human tongue.; western blot: 2-4 mug/mL using rat skeletal muscle extract
物种活性 :
rabbit, human, mouse, pig, bovine, rat, snake, cat, canine, frog, fish, goat, sheep, chicken, hamster, lizard
浓度 :
~1.0毫克/毫升
免疫原 :
purified rabbit skeletal alpha actinin.2
克隆 :
EA-53
应用 :
Western blot analysis of transfected HEK-293 cells were performed to evaluate actinin expression using monoclonal mouse anti-actinin antibody. 1
更多信息或购买 :
公司信息
西格玛奥德里奇
上海市徐汇区淮海中路1010号嘉华中心41楼
techservCN@sial.com
www.sigmaaldrich.com/china-mainland.html
800-819-3336 400-620-3333 86-21-6141 5566
公司总部: 美国
Sigma-Aldrich是一家在生命科学领域领先的且拥有高科技产品的公司,致力于为生命科学、制药化工等领域提供高质量的服务和产品。