产品简要
公司名称 :
西格玛奥德里奇
其他品牌 :
FLUKA
产品类型 :
抗体
产品名称 :
Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) antibody produced in mouse
目录 :
M9692
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
MAPK-YT

同一抗体克隆可从以下厂商购到:
  • 武汉博士德生物工程有限公司: MA1055
  • 亚诺法生技股份有限公司: MAB1463
反应物种 :
人类, 小鼠, 大鼠, , , 斑马鱼, 秀丽隐杆线虫
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-冰冻切片, reverse phase protein lysate microarray
更多信息或购买 :
文章摘录数: 25
已发表的文献中的应用方法/种属/稀释度参考文献
  • 免疫印迹; 小鼠; 图 1c
  • 免疫细胞化学; 小鼠; 图 1a
Kidger A, Rushworth L, Stellzig J, Davidson J, Bryant C, Bayley C, et al. Dual-specificity phosphatase 5 controls the localized inhibition, propagation, and transforming potential of ERK signaling. Proc Natl Acad Sci U S A. 2017;: pubmed 出版商
  • 免疫印迹; 猪; 图 1b
Ismail H, Didangelos A, Vincent T, Saklatvala J. Articular cartilage injury rapidly activates TAK1 in chondrocytes by inducing its phosphorylation and K63-polyubiquitination. Arthritis Rheumatol. 2016;: pubmed 出版商
  • 免疫印迹; 人类; 图 7e
Schütz I, López-Hernández T, Gao Q, Puchkov D, Jabs S, Nordmeyer D, et al. Lysosomal Dysfunction Caused by Cellular Accumulation of Silica Nanoparticles. J Biol Chem. 2016;291:14170-84 pubmed 出版商
  • 免疫印迹; 小鼠; 1:2000; 图 2
  • 免疫细胞化学; 小鼠; 1:500; 图 1
Sipieter F, Cappe B, Gonzalez Pisfil M, Spriet C, Bodart J, Cailliau-Maggio K, et al. Novel Reporter for Faithful Monitoring of ERK2 Dynamics in Living Cells and Model Organisms. PLoS ONE. 2015;10:e0140924 pubmed 出版商
  • 免疫印迹; 人类; 图 s5
Nan X, Tamgüney T, Collisson E, Lin L, Pitt C, Galeas J, et al. Ras-GTP dimers activate the Mitogen-Activated Protein Kinase (MAPK) pathway. Proc Natl Acad Sci U S A. 2015;112:7996-8001 pubmed 出版商
  • 免疫组化-冰冻切片; 斑马鱼; 1:50; 图 s5
Westcot S, Hatzold J, Urban M, Richetti S, Skuster K, Harm R, et al. Protein-Trap Insertional Mutagenesis Uncovers New Genes Involved in Zebrafish Skin Development, Including a Neuregulin 2a-Based ErbB Signaling Pathway Required during Median Fin Fold Morphogenesis. PLoS ONE. 2015;10:e0130688 pubmed 出版商
  • 免疫细胞化学; 大鼠; 1:250; 图 3
Maier P, Zemoura K, Acuña M, Yevenes G, Zeilhofer H, Benke D. Ischemia-like oxygen and glucose deprivation mediates down-regulation of cell surface γ-aminobutyric acidB receptors via the endoplasmic reticulum (ER) stress-induced transcription factor CCAAT/enhancer-binding protein (C/EBP)-homologous protein (CHOP). J Biol Chem. 2014;289:12896-907 pubmed 出版商
  • 免疫印迹; 人类
Chapnick D, Liu X. Leader cell positioning drives wound-directed collective migration in TGFβ-stimulated epithelial sheets. Mol Biol Cell. 2014;25:1586-93 pubmed 出版商
  • reverse phase protein lysate microarray; 人类; 1:500
Milara J, Peiró T, Serrano A, Guijarro R, Zaragozá C, Tenor H, et al. Roflumilast N-oxide inhibits bronchial epithelial to mesenchymal transition induced by cigarette smoke in smokers with COPD. Pulm Pharmacol Ther. 2014;28:138-48 pubmed 出版商
  • 免疫印迹; 小鼠; 1:500
Doughton G, Wei J, Tapon N, Welham M, Chalmers A. Formation of a polarised primitive endoderm layer in embryoid bodies requires fgfr/erk signalling. PLoS ONE. 2014;9:e95434 pubmed 出版商
  • 免疫印迹; 人类
Pastor-Clerigues A, Martí-Bonmatí E, Milara J, Almudever P, Cortijo J. Anti-inflammatory and anti-fibrotic profile of fish oil emulsions used in parenteral nutrition-associated liver disease. PLoS ONE. 2014;9:e115404 pubmed 出版商
Davaapil H, Brockes J, Yun M. Conserved and novel functions of programmed cellular senescence during vertebrate development. Development. 2017;144:106-114 pubmed 出版商
Schroter C, Rué P, Mackenzie J, Martinez Arias A. FGF/MAPK signaling sets the switching threshold of a bistable circuit controlling cell fate decisions in embryonic stem cells. Development. 2015;142:4205-16 pubmed 出版商
Ohta N, Satou Y. Multiple signaling pathways coordinate to induce a threshold response in a chordate embryo. PLoS Genet. 2013;9:e1003818 pubmed 出版商
Bickel R, Cleveland H, Barkas J, Jeschke C, Raz A, Stern D, et al. The pea aphid uses a version of the terminal system during oviparous, but not viviparous, development. Evodevo. 2013;4:10 pubmed 出版商
Lerner N, Beit-Yannai E. Cross-talk between ciliary epithelium and trabecular meshwork cells in-vitro: a new insight into glaucoma. PLoS ONE. 2014;9:e112259 pubmed 出版商
Schilling M, Maiwald T, Hengl S, Winter D, Kreutz C, Kolch W, et al. Theoretical and experimental analysis links isoform-specific ERK signalling to cell fate decisions. Mol Syst Biol. 2009;5:334 pubmed 出版商
Guidi M, Muiños-Gimeno M, Kagerbauer B, Marti E, Estivill X, Espinosa-Parrilla Y. Overexpression of miR-128 specifically inhibits the truncated isoform of NTRK3 and upregulates BCL2 in SH-SY5Y neuroblastoma cells. BMC Mol Biol. 2010;11:95 pubmed 出版商
Reischauer S, Levesque M, Nüsslein-Volhard C, Sonawane M. Lgl2 executes its function as a tumor suppressor by regulating ErbB signaling in the zebrafish epidermis. PLoS Genet. 2009;5:e1000720 pubmed 出版商
Shi L, Ko M, Abbott L, Ko G. Identification of Peptide lv, a novel putative neuropeptide that regulates the expression of L-type voltage-gated calcium channels in photoreceptors. PLoS ONE. 2012;7:e43091 pubmed 出版商
Eberhard R, Stergiou L, Hofmann E, Hofmann J, Haenni S, Teo Y, et al. Ribosome synthesis and MAPK activity modulate ionizing radiation-induced germ cell apoptosis in Caenorhabditis elegans. PLoS Genet. 2013;9:e1003943 pubmed 出版商
Leibovich-Rivkin T, Liubomirski Y, Meshel T, Abashidze A, Brisker D, Solomon H, et al. The inflammatory cytokine TNFα cooperates with Ras in elevating metastasis and turns WT-Ras to a tumor-promoting entity in MCF-7 cells. BMC Cancer. 2014;14:158 pubmed 出版商
Heger P, Kroiher M, Ndifon N, Schierenberg E. Conservation of MAP kinase activity and MSP genes in parthenogenetic nematodes. BMC Dev Biol. 2010;10:51 pubmed 出版商
Gonzalez-Garcia J, Bradley J, Nomikos M, Paul L, Machaty Z, Lai F, et al. The dynamics of MAPK inactivation at fertilization in mouse eggs. J Cell Sci. 2014;127:2749-60 pubmed 出版商
Sugden P, Markou T, Fuller S, Tham E, Molkentin J, Paterson H, et al. Monophosphothreonyl extracellular signal-regulated kinases 1 and 2 (ERK1/2) are formed endogenously in intact cardiac myocytes and are enzymically active. Cell Signal. 2011;23:468-77 pubmed 出版商
产品信息
目录号 :
M9692
品牌 :
SIGMA
名称 :
Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) antibody produced in mouse
产品类型 :
化学品
名字后缀 :
1.5-2 mg/mL, clone MAPK-YT, purified immunoglobulin, buffered aqueous solution
储存 :
-20C
储存温度 :
-20C
物理形态 :
溶液在磷酸盐缓冲液, pH值7.4, 含15 mM叠氮化钠
运输的 :
干冰
同义词 :
Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2)
unspsc code :
12352203
wgk :
nwg
形式 :
buffered aqueous solution
抗体形态 :
纯化免疫球蛋白
应用(s) :
indirect ELISA: suitable; immunoprecipitation: suitable; immunocytochemistry: suitable; western blot: 0.5-1 mug/mL using a whole cell extract of RAT-1 cells treated with vanadate and H2O2.; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; microarray: suitable
物种活性 :
酵母, 蛙, 人类, 大鼠, 牛, 秀丽隐杆线虫, 果蝇, 小鼠, 仓鼠
浓度 :
1.5-2毫克/毫升
免疫原 :
synthetic peptide HTGFLpTEpYVAT corresponding to the phosphorylated form of the ERK-activation loop.
克隆 :
MAPK-YT
分子量 :
antigen ERK-2 mol wt 42 kDa; antigen ERK-1 mol wt 44 kDa
更多信息或购买 :
公司信息
西格玛奥德里奇
上海市徐汇区淮海中路1010号嘉华中心41楼
techservCN@sial.com
www.sigmaaldrich.com/china-mainland.html
800-819-3336 400-620-3333 86-21-6141 5566
公司总部: 美国
Sigma-Aldrich是一家在生命科学领域领先的且拥有高科技产品的公司,致力于为生命科学、制药化工等领域提供高质量的服务和产品。