蛋白

AKT1, 活性的

A16-10G

5微克/10微克

145/205美元

AKT1, 活性的

活性的激酶

Sf9昆虫细胞美国杆状病毒

谷胱甘肽S-转移酶

5微克/10微克

145/205美元

NM_005163

Recombinant full-length human AKT1 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

人类

AKT1/PKBα is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2).

PKB;RAC;PRKBA;MGC99656;RAC-甲

>90%

50mM三羟甲基氨基甲烷氯化氢, pH值7.5, 150mM氯化钠, 10mM谷胱甘肽, 0.1mM乙二胺四乙酸, 0.25mM DTT, 0.1mM苯甲基磺酰氟, 25% 甘油

干冰

0.1微克/微升

Kinase Assay, Western Blot

The specific activity was determined to be 125 nmol/min/mg as per activity assay protocol.

Store product at –70ºC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.