蛋白

ERK2, 活性的

M28-10G

5微克/10微克

205/295美元

ERK2, 活性的

活性的激酶

大肠杆菌

/

谷胱甘肽S-转移酶

5微克/10微克

205/295美元

NM_002745

Recombinant full-length human ERK2 was expressed by E. coli cells using an N-terminal GST tag and activated by MEK1 in vitro.

人类

ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK2 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK2 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK2 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).

MAPK1, 丝裂原活化蛋白激酶P42, MAPK2, PRKM2, PRKM1, p41mapk, ERT1, p40, p41, ERK

>95%

50mM三羟甲基氨基甲烷氯化氢, pH值7.5, 150mM氯化钠, 0.25mM DTT, 0.1mM乙二醇-双-(2-氨基乙醚)四乙酸, 0.1mM乙二胺四乙酸, 0.1mM苯甲基磺酰氟, 25% 甘油

干冰

0.1微克/微升

Kinase Assay, Western Blot

The specific activity was determined to be 682 nmol/min/mg as per activity assay protocol.

Store product at –70ºC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.