蛋白

p53蛋白

P05-30BG

20微克/50微克

195/395美元

p53蛋白

细胞周期蛋白

Sf9昆虫细胞美国杆状病毒

谷胱甘肽S-转移酶

20微克/50微克

195/395美元

NM_000546

Recombinant full-length human p53 was expressed by baculovirus in Hi5 cells using an N-terminal GST tag.

人类

p53 is a key regulator of cell growth and acts as a tumor suppressor gene. Wild-type p53 gene can suppress transformation of rat embryo fibroblasts in cell culture by other oncogenes such as the adenovirus E1A and Ras (1). DNA tumor viruses such as SV40 large T antigen and the adenovirus E1A plus E1B-55Kd proteins bind to p53 and inactivate its tumor suppressor activities leading to cellular transformation. Mutational inactivation of the p53 gene is detected in more than 50% of human cancers. Mutation of p53 renders cancer cells more resistant to current cancer therapies due to lack of p53-mediated apoptosis (2).

TP53, LFS1, TRP53

>95%

50mM三羟甲基氨基甲烷氯化氢, pH值7.5, 50mM氯化钠, 0.25mM DTT, 0.1mM乙二胺四乙酸, 0.1mM苯甲基磺酰氟, 10mM谷胱甘肽, 25% 甘油

干冰

0.2微克/微升

Kinase Assay, Western Blot

Store product at –70ºC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.