产品简要
公司名称 :
北京义翘神州
产品类型 :
蛋白
产品名称 :
人类HER2/v-erb-b2成红细胞白血病病毒致瘤基因2蛋白(多聚组氨酸标签)
目录 :
10004-H08H
规格 :
100微克
价格 :
348美元
更多信息或购买 :
北京义翘神州 产品网页
文章摘录数: 29
出版应用/物种/样本/稀释参考文献
  • 酶联免疫吸附测定; 人类
Murakami S, Matsumoto R, Kanamori T. Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system. Sci Rep. 2019;9:671 pubmed 出版商
  • 酶联免疫吸附测定; 人类
Albers A, Garofalo A, Drake P, Kudirka R, de Hart G, Barfield R, et al. Exploring the effects of linker composition on site-specifically modified antibody-drug conjugates. Eur J Med Chem. 2014;88:3-9 pubmed 出版商
Conilh L, Fournet G, Fourmaux E, Murcia A, Matera E, Joseph B, et al. Exatecan Antibody Drug Conjugates Based on a Hydrophilic Polysarcosine Drug-Linker Platform. Pharmaceuticals (Basel). 2021;14: pubmed 出版商
Shi R, Xiao G, Dillon T, McAuley A, Ricci M, Bondarenko P. Identification of critical chemical modifications by size exclusion chromatography of stressed antibody-target complexes with competitive binding. MAbs. 2021;13:1887612 pubmed 出版商
Leeman M, Albers W, Bombera R, Kuncová Kallio J, Tuppurainen J, Nilsson L. Asymmetric flow field-flow fractionation coupled to surface plasmon resonance detection for analysis of therapeutic proteins in blood serum. Anal Bioanal Chem. 2021;413:117-127 pubmed 出版商
Pekar L, Busch M, Valldorf B, Hinz S, Toleikis L, Krah S, et al. Biophysical and biochemical characterization of a VHH-based IgG-like bi- and trispecific antibody platform. MAbs. 2020;12:1812210 pubmed 出版商
Bansal R, Elgundi Z, Goodchild S, Care A, Lord M, Rodger A, et al. The Effect of Oligomerization on A Solid-Binding Peptide Binding to Silica-Based Materials. Nanomaterials (Basel). 2020;10: pubmed 出版商
Klichinsky M, Ruella M, Shestova O, Lu X, Best A, Zeeman M, et al. Human chimeric antigen receptor macrophages for cancer immunotherapy. Nat Biotechnol. 2020;38:947-953 pubmed 出版商
Kim J, Suh J, Yang J. Labeling-free detection of ECD-HER2 protein using aptamer-based nano-plasmonic sensor. Nanotechnology. 2020;31:175501 pubmed 出版商
Ramos J, Vega J, Cruz V, Sánchez Sánchez E, Cortes J, Martinez Salazar J. Hydrodynamic and Electrophoretic Properties of Trastuzumab/HER2 Extracellular Domain Complexes as Revealed by Experimental Techniques and Computational Simulations. Int J Mol Sci. 2019;20: pubmed 出版商
Hu F, Lundqvist M, Uhlen M, Rockberg J. SAMURAI (Solid-phase Assisted Mutagenesis by Uracil Restriction for Accurate Integration) for antibody affinity maturation and paratope mapping. Nucleic Acids Res. 2019;47:e34 pubmed 出版商
Lombana T, Matsumoto M, Berkley A, Toy E, Cook R, Gan Y, et al. High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies. MAbs. 2019;11:75-93 pubmed 出版商
Su C, Lua W, Ling W, Gan S. Allosteric Effects between the Antibody Constant and Variable Regions: A Study of IgA Fc Mutations on Antigen Binding. Antibodies (Basel). 2018;7: pubmed 出版商
Huang S, Li F, Liu H, Ye P, Fan X, Yuan X, et al. Structural and functional characterization of MBS301, an afucosylated bispecific anti-HER2 antibody. MAbs. 2018;10:864-875 pubmed 出版商
Hollevoet K, De Smidt E, Geukens N, Declerck P. Prolonged in vivo expression and anti-tumor response of DNA-based anti-HER2 antibodies. Oncotarget. 2018;9:13623-13636 pubmed 出版商
Yu F, Alesand V, Nygren P. Site-Specific Photoconjugation of Beta-Lactamase Fragments to Monoclonal Antibodies Enables Sensitive Analyte Detection via Split-Enzyme Complementation. Biotechnol J. 2018;13:e1700688 pubmed 出版商
Lua W, Ling W, Yeo J, Poh J, Lane D, Gan S. The effects of Antibody Engineering CH and CL in Trastuzumab and Pertuzumab recombinant models: Impact on antibody production and antigen-binding. Sci Rep. 2018;8:718 pubmed 出版商
Lua W, Gan S, Lane D, Verma C. A search for synergy in the binding kinetics of Trastuzumab and Pertuzumab whole and F(ab) to Her2. NPJ Breast Cancer. 2015;1:15012 pubmed 出版商
Vega J, Ramos J, Cruz V, Vicente Alique E, Sánchez Sánchez E, Sánchez Fernández A, et al. Molecular and hydrodynamic properties of human epidermal growth factor receptor HER2 extracellular domain and its homodimer: Experiments and multi-scale simulations. Biochim Biophys Acta Gen Subj. 2017;1861:2406-2416 pubmed 出版商
Su C, Ling W, Lua W, Poh J, Gan S. The role of Antibody Vκ Framework 3 region towards Antigen binding: Effects on recombinant production and Protein L binding. Sci Rep. 2017;7:3766 pubmed 出版商
Aubé A, Campbell S, Schmitzer A, Claing A, Masson J. Ultra-low fouling alkylimidazolium modified surfaces for the detection of HER2 in breast cancer cell lysates [corrected]. Analyst. 2017;142:2343-2353 pubmed 出版商
Eletxigerra U, Martinez Perdiguero J, Barderas R, Pingarrón J, Campuzano S, Merino S. Surface plasmon resonance immunosensor for ErbB2 breast cancer biomarker determination in human serum and raw cancer cell lysates. Anal Chim Acta. 2016;905:156-62 pubmed 出版商
Nilvebrant J, Astrand M, Georgieva Kotseva M, Björnmalm M, Löfblom J, Hober S. Engineering of bispecific affinity proteins with high affinity for ERBB2 and adaptable binding to albumin. PLoS ONE. 2014;9:e103094 pubmed 出版商
Gilabert Oriol R, Thakur M, von Mallinckrodt B, Hug T, Wiesner B, Eichhorst J, et al. Modified trastuzumab and cetuximab mediate efficient toxin delivery while retaining antibody-dependent cell-mediated cytotoxicity in target cells. Mol Pharm. 2013;10:4347-57 pubmed 出版商
Kim K, Song J, Kee J, Liu Q, Lo G, Park M. Label-free biosensor based on an electrical tracing-assisted silicon microring resonator with a low-cost broadband source. Biosens Bioelectron. 2013;46:15-21 pubmed 出版商
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Liu Z, Duan J, Song Y, Ma J, Wang F, Lu X, et al. Novel HER2 aptamer selectively delivers cytotoxic drug to HER2-positive breast cancer cells in vitro. J Transl Med. 2012;10:148 pubmed 出版商
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图像
图像 1 :
北京义翘神州 10004-H08H 图像 1
产品信息
提示 :
Online
目录代码 :
10004-H08H
产品名称 :
人类HER2/v-erb-b2成红细胞白血病病毒致瘤基因2蛋白(多聚组氨酸标签)
产品类型 :
蛋白
宿主物种 :
HEK293细胞
共轭 :
C多聚组氨酸
规格 :
100微克
标价 :
348美元
基因编号 :
NP_004439.2
产品描述 :
A DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ErbB2 (NP_004439.2) was fused with the polyhistidine tag at the C-terminus.
物种摘要 :
人类
别名 :
v-erb-b2成红细胞白血病病毒致瘤基因2
特异性 :
1. Measured by its binding ability in a functional ELISA. Immobilized human Erbb2 at 2.5 μg/ml (100 μl/well) can bind Herceptin with a linear range of 1.28-32 ng/ml. 2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.2-1 μg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody. 3. Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant (His Tag) (Cat: 10004-H08H) bound to Pertuzumab was 0.1 nM.
纯度 :
> 90 % as determined by SDS-PAGE
缓冲液 :
Lyophilized from sterile PBS, pH 7.4
包装 :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
储存 :
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
更多信息或购买 :
北京义翘神州 产品网页
公司信息
北京义翘神州
北京市北京经济技术开发区科创十街18号院9号楼
Order@Sinobiological.com
http://www.sinobiological.cn
400-890-9989
公司总部: 中国
北京义翘神州科技股份有限公司(Sino Biological Inc.)是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务有重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。

义翘科技为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。义翘科技目前生产和销售的现货产品种类超过4.6万种，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求，并覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供“一站式”生物试剂产品和技术服务。

义翘科技的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，累计客户数量超过5,000个，品牌声誉不断累积，已成为生物试剂行业国内领先的科技公司之一。