Human CDC2 & CCNE1 Heterotrimer Protein
NP_001777.1 & NP_001229.1
A DNA sequence encoding the human CDC2 (NP_001777.1) (Met1-Met297) was expressed with a GST tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNE1 (NP_001229.1) (Met1-Ala410) was expressed with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterotrimer was purified.
> 90 % as determined by SDS-PAGE
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 8.0.
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.